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Gene Review:

bocksbeutel - CG9424 gene product from transcript CG9424-RA

Drosophila melanogaster

Synonyms: Bocks, Bocksbeutel, CG9424, Dmel\CG9424, emerin

Wagner, N. et al., Wagner, N. et al.

Wagner, Kagermeier, Loserth, Krohne,

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High impact information on bocksbeutel

Downregulation of dMAN1 by RNA interference (RNAi) in Drosophila cultured Kc167 cells has no obvious effect on nuclear architecture, viability of RNAi-treated cells and the intracellular distribution of the LEM-domain proteins Bocksbeutel and otefin [1].
In contrast, the localization of dMAN1, Bocksbeutel and otefin at the INM is supported by lamin Dm0 [1].
Indirect immunofluorescence with Bocksbeutel-specific antibodies on tissues and cultured cells revealed that Bocksbeutel proteins are localized in the nuclear envelope and in the cytoplasm [2].
The down-regulation of Bocksbeutel and otefin had no influence on the viability of Kc167 cells and the intracellular localization of all other nuclear and nuclear envelope proteins analyzed [2].
The Bocksbeutel gene can code for two isoforms of 399 and 351 amino acids that are produced by alternative splicing [2].

Biological context of bocksbeutel

By RNA interference we have down-regulated the expression of Bocksbeutel, BAF, otefin, and lamin DmO in Drosophila Kc167 cells [2].
We conclude that the two LEM-domain proteins Bocksbeutel and otefin are no limiting components for the maintenance of the nuclear architecture in cultured Drosophila cells at interphase [2].

Anatomical context of bocksbeutel

When expressed in transfected cells as GFP fusion proteins, the beta-isoform is localized predominantly in the nucleoplasm and the alpha-isoform is targeted to the nuclear envelope, indicating that Bocksbeutel-alpha is localized in the inner nuclear membrane [2].

Other interactions of bocksbeutel

Of all putative Drosophila LEM-domain proteins, otefin and Bocksbeutel exhibited the highest similarity in the LEM motif (53% identical amino acids) [2].
In contrast, when lamin DmO was reduced by RNAi the distribution of Bocksbeutel and otefin in the nuclear envelope of Kc167 cells was significantly altered [2].

References

The Drosophila melanogaster LEM-domain protein MAN1. Wagner, N., Kagermeier, B., Loserth, S., Krohne, G. Eur. J. Cell Biol. (2006) [Pubmed]
Two novel LEM-domain proteins are splice products of the annotated Drosophila melanogaster gene CG9424 (Bocksbeutel). Wagner, N., Schmitt, J., Krohne, G. Eur. J. Cell Biol. (2004) [Pubmed]

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