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Gene Review:

dup - double parked

Drosophila melanogaster

Synonyms: 19k, CDT1, CDT1/DUP, CG8171, Cdt1, ...

Mihaylov, I.S. et al., May, N.R. et al., Whittaker, A.J. et al., Smith, A.V. et al., Garner, M. et al.

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High impact information on dup

Interestingly, dup mutant embryos fail both to downregulate S phase genes and to engage a checkpoint preventing mitosis until completion of S phase [1].
We identified a Drosophila gene, double parked (dup), that is essential for DNA replication and belongs to a new family of replication proteins conserved from Schizosaccharomyces pombe to humans [1].
We observed that the silencing of geminin causes rapid downregulation of Cdt1/Dup, which may contribute to the observed partial overreplication in geminin-deficient cells [2].
We find that mitotic cyclins are stabilized and that a spindle checkpoint protein, Bub1, localizes onto chromosomes during mitotic arrest in dup mutants [3].
We were able to identify a single, lethal complementation group in 51E, l(2)51Ec, that is responsible for the cessation of replication observed in this interval [4].

Regulatory relationships of dup

Cdt1 is an essential component of the pre-RC that is rapidly degraded at G1-S and also inhibited by Geminin (Gem) protein to prevent re-replication [5].

Other interactions of dup

Levels of the origin-binding protein Double parked and its inhibitor Geminin increase in response to replication stress [5].

References

Drosophila double parked: a conserved, essential replication protein that colocalizes with the origin recognition complex and links DNA replication with mitosis and the down-regulation of S phase transcripts. Whittaker, A.J., Royzman, I., Orr-Weaver, T.L. Genes Dev. (2000) [Pubmed]
Control of DNA replication and chromosome ploidy by geminin and cyclin A. Mihaylov, I.S., Kondo, T., Jones, L., Ryzhikov, S., Tanaka, J., Zheng, J., Higa, L.A., Minamino, N., Cooley, L., Zhang, H. Mol. Cell. Biol. (2002) [Pubmed]
mei-41 and bub1 block mitosis at two distinct steps in response to incomplete DNA replication in Drosophila embryos. Garner, M., van Kreeveld, S., Su, T.T. Curr. Biol. (2001) [Pubmed]
Identification of genomic regions required for DNA replication during Drosophila embryogenesis. Smith, A.V., King, J.A., Orr-Weaver, T.L. Genetics (1993) [Pubmed]
Levels of the origin-binding protein Double parked and its inhibitor Geminin increase in response to replication stress. May, N.R., Thomer, M., Murnen, K.F., Calvi, B.R. J. Cell. Sci. (2005) [Pubmed]

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AgaP_AGAP005321	Anopheles gambiae str. PEST
LOC711530	Macaca mulatta

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