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Gene Review:

PFN2 - profilin 2

Bos taurus

Lambrechts, A. et al., Lambrechts, A. et al., Jonckheere, V. et al.

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High impact information on LOC539034

Using affinity chromatography on either profilin isoform, we identified profilin II as the preferred ligand for VASP in bovine brain extracts [1].
At micromolar concentrations, profilin II dimerizes upon binding to proline-rich peptides [1].
Using microfiltration, we show that compared with profilin II, profilin I has a higher affinity for PIP2 [1].
Profilin II dimers bind the (GP5)3 peptide derived from VASP with an affinity of approximately 0.5 microM [2].
The resulting profilin II-peptide complex overcomes the combined capacity of thymosin beta4 and profilin II to inhibit actin nucleation and restores the extent of filament formation [2].

Associations of LOC539034 with chemical compounds

Purification and characterization of bovine profilin II. Actin, poly(L-proline) and inositolphospholipid binding [3].
Profilin II also binds the anionic phospholipid phosphatidylinositol 4,5-bisphosphate [3].

Physical interactions of LOC539034

Profilin II binds to poly(L-proline) more strongly than does profilin I; this is especially evident at more acidic pH values [3].

Other interactions of LOC539034

We show further that PIP2 effectively competes for binding of profilin I to poly-L-proline, since this isoform, but not profilin II, can be eluted from a poly-L-proline column with PIP2 [1].

Analytical, diagnostic and therapeutic context of LOC539034

Circular dichroism measurements of profilin II reveal a significant conformational change in this protein upon binding of the peptide [1].

References

The mammalian profilin isoforms display complementary affinities for PIP2 and proline-rich sequences. Lambrechts, A., Verschelde, J.L., Jonckheere, V., Goethals, M., Vandekerckhove, J., Ampe, C. EMBO J. (1997) [Pubmed]
Dimerization of profilin II upon binding the (GP5)3 peptide from VASP overcomes the inhibition of actin nucleation by profilin II and thymosin beta4. Jonckheere, V., Lambrechts, A., Vandekerckhove, J., Ampe, C. FEBS Lett. (1999) [Pubmed]
Purification and characterization of bovine profilin II. Actin, poly(L-proline) and inositolphospholipid binding. Lambrechts, A., van Damme, J., Goethals, M., Vandekerckhove, J., Ampe, C. Eur. J. Biochem. (1995) [Pubmed]

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pfn2l	Danio rerio
PFN2	Gallus gallus
PFN2	Homo sapiens
LOC713460	Macaca mulatta
Pfn2	Mus musculus
PFN2	Pan troglodytes
LOC100909840	Rattus norvegicus
Pfn2	Rattus norvegicus
pfn2	Xenopus (Silurana) tropicalis

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