Disease relevance of SAA3P

• In addition to its role in regulating SAA3 gene expression, we provide evidence that SEF could also bind in a sequence-specific manner to the promoters of the alpha(2)-macroglobulin and Aalpha-fibrinogen genes and to an intronic enhancer of the human Wilm's tumor 1 gene, suggesting a functional role in the regulation of these genes [1].

High impact information on SAA3P

• We report here the characterization of a cell line derived from mouse liver (BNL) in which the expression of SAA3 mRNA is regulated [2].
• These observations suggest that conditioned medium contains a factor(s) that acts directly on hepatocytes to regulate SAA3 mRNA processing [2].
• In this model, SAA3 mRNA accumulated in response to conditioned medium from the mouse macrophage P388D1 cell line with kinetics similar to that seen in mouse liver (C. A. Lowell, R. S. Stearman, and J. F. Morrow, J. Biol. Chem. 261:8453-8461, 1986) [2].
• The acute-phase reactant rabbit serum amyloid A 3 (SAA3) was identified as the major difference product in Ag-induced arthritis in the rabbit, a model resembling in many aspects the clinical characteristics of rheumatoid arthritis (RA) in humans [3].
• The proinflammatory cytokine IL-1beta induced SAA3 transcription in primary rabbit chondrocytes in vitro [3].

Biological context of SAA3P

• A fourth locus, SAA3, is a pseudogene [4].
• 1. To order SAA3 on the genetic map, a highly polymorphic (CA)n dinucleotide repeat within SAA3 was typed through the CEPH reference families [5].

Anatomical context of SAA3P

• In Ag-induced arthritis, up-regulated SAA3 transcription in vivo was detected in cells infiltrating into the inflamed joint, in the area where pannus formation starts and, most notably, also in chondrocytes [3].

Other interactions of SAA3P

• SAA3 is 110 kb downstream of SAA4, and its relative orientation could not be determined [4].
• The putative fifth human serum amyloid A protein (SAA)-related gene "SAA5" is defined by SAA3 [6].
• To further elucidate its role in the regulation of the SAA3 gene, we purified SEF from HeLa nuclear extracts to near homogeneity by using conventional liquid chromatography and DNA affinity chromatography [1].

References