High impact information on LEP

• These results suggest LEP is a positive regulator of GA-induced germination acting independently of RGL2 [1].
• In addition, lep-1 is less responsive to GA and more sensitive to the GA biosynthesis inhibitor paclobutrazol, indicating that LEP is a positive regulator of GA-induced germination [1].
• A new role for the Arabidopsis AP2 transcription factor, LEAFY PETIOLE, in gibberellin-induced germination is revealed by the misexpression of a homologous gene, SOB2/DRN-LIKE [1].
• Therefore, LEP is involved in either cell division activity in the marginal meristem or patterning along the proximodistal axis [2].
• The LEAFY PETIOLE (LEP) gene is located close to the right border of the T-DNA insert linked with these dominant phenotypes and encodes a protein with a domain with similarity to the DNA binding domain of members of the AP2/EREBP family of transcription factors [2].

Biological context of LEP

• We conclude that activation tagging of LEP and VAS results in additive phenotypes [3].
• Activation tagging of the two closely linked genes LEP and VAS independently affects vascular cell number [3].
• In order to isolate mutants that are specifically disturbed in the outgrowth of the leaf blade, second site mutagenesis was performed using ethane methanesulphonate (EMS) on a transgenic line that harbours the activation-tagged LEP gene and exhibits the leafy petiole phenotype [4].

Other interactions of LEP

• An alternative model places LEP downstream of RGL2 in the GA-signaling cascade [1].

Analytical, diagnostic and therapeutic context of LEP

• RT-PCR shows that LEP transcript accumulates in wild-type seeds during imbibition and germination, and the transcript levels of REPRESSOR OF ga1-3-LIKE2 (RGL2), a negative regulator of GA signaling during germination, is unaffected in lep-1 [1].

References