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Gene Review

MDH3  -  malate dehydrogenase MDH3

Saccharomyces cerevisiae S288c

Synonyms: D2468, Malate dehydrogenase, peroxisomal, YDL078C
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High impact information on MDH3

  • In addition, mdh3-disrupted cells were unable to grow on oleate whereas growth on other non-fermentable carbon sources was normal, suggesting that MDH3 is involved in the reoxidation of NADH generated during fatty acid beta-oxidation rather than functioning as part of the glyoxylate cycle [1].
  • A randomly mutagenized pex5 library was screened in a two-hybrid setup for mutations that disrupted the interaction with the PTS1 protein Mdh3p or for suppressor mutations that could restore the interaction with Mdh3p containing a mutation in its PTS1 [2].
  • The MDH3 isozyme of Saccharomyces cerevisiae was purified from a haploid strain containing disruptions in genomic loci encoding the mitochondrial MDH1 and nonmitochondrial MDH2 isozymes [3].
  • The MDH3 sequence was found to contain a carboxyl-terminal SKL tripeptide, characteristic of many peroxisomal enzymes, and immunochemical analysis was used to confirm organellar localization of the MDH3 isozyme [3].
  • Partial amino acid sequence analysis of the purified enzyme was conducted and used to plan polymerase chain reaction techniques to clone the MDH3 gene [3].

Associations of MDH3 with chemical compounds

  • Disruption of the chromosomal MDH3 locus produced a reduction in cellular growth rates on acetate, consistent with the presumed function of this isozyme in the glyoxylate pathway of yeast [3].

Other interactions of MDH3

  • This TCA cycle function observed for the cytosolic form of MDH1 is unique to that isozyme since overexpression of MDH2 and of a cytosolic form of MDH3 in a deltaMDH1 strain failed to restore growth [4].


  1. The membrane of peroxisomes in Saccharomyces cerevisiae is impermeable to NAD(H) and acetyl-CoA under in vivo conditions. van Roermund, C.W., Elgersma, Y., Singh, N., Wanders, R.J., Tabak, H.F. EMBO J. (1995) [Pubmed]
  2. Recognition of peroxisomal targeting signal type 1 by the import receptor Pex5p. Klein, A.T., Barnett, P., Bottger, G., Konings, D., Tabak, H.F., Distel, B. J. Biol. Chem. (2001) [Pubmed]
  3. Isolation and characterization of the yeast gene encoding the MDH3 isozyme of malate dehydrogenase. Steffan, J.S., McAlister-Henn, L. J. Biol. Chem. (1992) [Pubmed]
  4. Metabolic effects of altering redundant targeting signals for yeast mitochondrial malate dehydrogenase. Small, W.C., McAlister-Henn, L. Arch. Biochem. Biophys. (1997) [Pubmed]
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