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Gene Review:

MDH2 - malate dehydrogenase MDH2

Saccharomyces cerevisiae S288c

Synonyms: Malate dehydrogenase, cytoplasmic, YOL126C

Minard, K.I. et al., Minard, K.I. et al., Small, W.C. et al., Asano, T. et al., Steffan, J.S. et al.

Asano, Kurose, Tarumi,

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High impact information on MDH2

The deduced amino acid sequence was closely related to those of MDH1 (50% residue identity) and of MDH2 (43% residue identity) [1].
Glucose-induced degradation of the MDH2 isozyme of malate dehydrogenase in yeast [2].
An extended lag in attaining wild type rates of growth on glucose measured for strains expressing the truncated MDH2 enzyme represents the first evidence of a selective advantage for the phenomenon of glucose-induced proteolysis in yeast [2].
A truncated form of MDH2 lacking amino acid residues 1-12 was constructed by mutagenesis of the MDH2 gene and expressed in a haploid yeast strain containing a deletion disruption of the corresponding chromosomal gene [2].
This TCA cycle function observed for the cytosolic form of MDH1 is unique to that isozyme since overexpression of MDH2 and of a cytosolic form of MDH3 in a deltaMDH1 strain failed to restore growth [3].

Biological context of MDH2

Glucose-induced phosphorylation of the MDH2 isozyme of malate dehydrogenase in Saccharomyces cerevisiae [4].

Associations of MDH2 with chemical compounds

However, the transcriptional level of the MDH2 gene in strain M20 for days 4 and 8 of malate formation during sake brewing was higher than that in strain K-701 [5].
Shifting a yeast culture growing with acetate to medium containing glucose as a carbon source resulted in a 25-fold increase in turnover of MDH2 [2].

Enzymatic interactions of MDH2

In this report, we show that MDH2 is phosphorylated during the process of glucose-induced degradation [4].

References

Isolation and characterization of the yeast gene encoding the MDH3 isozyme of malate dehydrogenase. Steffan, J.S., McAlister-Henn, L. J. Biol. Chem. (1992) [Pubmed]
Glucose-induced degradation of the MDH2 isozyme of malate dehydrogenase in yeast. Minard, K.I., McAlister-Henn, L. J. Biol. Chem. (1992) [Pubmed]
Metabolic effects of altering redundant targeting signals for yeast mitochondrial malate dehydrogenase. Small, W.C., McAlister-Henn, L. Arch. Biochem. Biophys. (1997) [Pubmed]
Glucose-induced phosphorylation of the MDH2 isozyme of malate dehydrogenase in Saccharomyces cerevisiae. Minard, K.I., McAlister-Henn, L. Arch. Biochem. Biophys. (1994) [Pubmed]
Isolation of high-malate-producing sake yeasts from low-maltose-assimilating mutants. Asano, T., Kurose, N., Tarumi, S. J. Biosci. Bioeng. (2001) [Pubmed]

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