Disease relevance of NUP42

• It contributes to HIV-1 Rev-mediated RNA export and is also important for the export of heat shock RNAs at 42 degrees C. We show here that Rip1p is essential for the export of heat shock RNAs, and this function is fulfilled by the unique carboxyl terminus of Rip1p with no substantial contribution from the FG-repeat region [1].

High impact information on NUP42

• Gle1 interacts with Rip1 and the nucleoporin Nup100 and is localized predominantly at nuclear pore complexes [2].
• The export of Rev itself proceeds through the nuclear pore complex and requires a nuclear export signal (NES) and interaction with a cellular cofactor, the protein Rip1 [2].
• When SSA4 mRNA was induced from a GAL promoter in the absence of stress, it was efficiently exported in a strain lacking RIP1, indicating that Rip1p is required for export of heat shock mRNAs only following stress [3].
• Rip1p is a 42-kD protein associated with nuclear pore complexes and contains nucleoporin-like repeat sequences [3].
• Export following ethanol shock was less dependent on the presence of Rip1p [4].

Biological context of NUP42

• Interestingly, a two-hybrid interaction was detected between Gle2p and Srp1p, the nuclear localization signal receptor, as well as Rip1p, a nuclear export signal-interacting protein [5].

Physical interactions of NUP42

• Rip1p interacts directly with Gle1p and is the only protein known to be essential for mRNA export after heat shock but not under normal growth conditions [4].

Other interactions of NUP42

• With Nup42 and Nup159 localized exclusively to the nuclear pore complex cytoplasmic side, we speculated that IP6 may regulate a cytoplasmic step in mRNA export [6].
• Genetic interactions between Rip1p and the RNA export mediator Gle1p are described, which support a role of the carboxyl terminus of Rip1p in poly(A)+ RNA export [1].

Analytical, diagnostic and therapeutic context of NUP42

• The FG-repeat domain of the yeast Rip1 protein (Rip1p) was identified initially as a possible target for the nuclear export signal (NES) of the HIV-1 Rev protein in a yeast two-hybrid assay [1].

References