Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

NUP100 - Nup100p

Saccharomyces cerevisiae S288c

Synonyms: NSP100, Nuclear pore protein NUP100/NSP100, Nucleoporin NUP100/NSP100, YKL068W, YKL336

Wente, S.R. et al., Iovine, M.K. et al., Rasmussen, S.W., Nazarenus, T. et al., Strawn, L.A. et al., et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on NUP100

Gle1 interacts with Rip1 and the nucleoporin Nup100 and is localized predominantly at nuclear pore complexes [1].
Nup116p and nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor gle2p [2].
A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p [3].
In contrast, the NES mutation abolished Kap95p interaction with the GLFG repeat regions from the nucleoporins Nup116p and Nup100p [3].
DNA sequence analysis has shown that the amino-terminal regions of NUP49, NUP100, and NUP116 share repeated "GLFG" motifs separated from each other by glutamine, asparagine, serine and threonine rich spacers [4].

Biological context of NUP100

NUP116-deficient cells grow very slowly and are temperature sensitive, whereas the lack of NUP100 has no detectable phenotype [4].
Interestingly, NUP100 and NUP116 are both flanked by a histidine tRNA gene and a transposon element suggesting that they may have arisen by gene duplication [4].
Sequence of a 20.7 kb region of yeast chromosome XI includes the NUP100 gene, an open reading frame (ORF) possibly representing a nucleoside diphosphate kinase gene, tRNAs for His, Val and Trp in addition to seven ORFs with weak or no significant similarity to known proteins [5].

Anatomical context of NUP100

We have identified a two-hybrid interaction between Upf1p and the nuclear pore (Nup) proteins, Nup100p and Nup116p [6].

Associations of NUP100 with chemical compounds

The deletion of NUP100 allows upf1Delta, can1-100 cells to grow in the presence of canavanine [6].

Physical interactions of NUP100

We have previously shown that the GLFG regions of Nup116p and Nup100p directly bind the karyopherin transport factor Kap95p during nuclear protein import [7].

Other interactions of NUP100

Finally, we see a genetic interaction between UPF1 and NUP100 [6].

References

An RNA-export mediator with an essential nuclear export signal. Murphy, R., Wente, S.R. Nature (1996) [Pubmed]
Nup116p and nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor gle2p. Bailer, S.M., Siniossoglou, S., Podtelejnikov, A., Hellwig, A., Mann, M., Hurt, E. EMBO J. (1998) [Pubmed]
A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p. Iovine, M.K., Wente, S.R. J. Cell Biol. (1997) [Pubmed]
A new family of yeast nuclear pore complex proteins. Wente, S.R., Rout, M.P., Blobel, G. J. Cell Biol. (1992) [Pubmed]
Sequence of a 20.7 kb region of yeast chromosome XI includes the NUP100 gene, an open reading frame (ORF) possibly representing a nucleoside diphosphate kinase gene, tRNAs for His, Val and Trp in addition to seven ORFs with weak or no significant similarity to known proteins. Rasmussen, S.W. Yeast (1994) [Pubmed]
Upf1p, a highly conserved protein required for nonsense-mediated mRNA decay, interacts with the nuclear pore proteins Nup100p and Nup116p. Nazarenus, T., Cedarberg, R., Bell, R., Cheatle, J., Forch, A., Haifley, A., Hou, A., Wanja Kebaara, B., Shields, C., Stoysich, K., Taylor, R., Atkin, A.L. Gene (2005) [Pubmed]
The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex. Strawn, L.A., Shen, T., Wente, S.R. J. Biol. Chem. (2001) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.