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Gene Review

GLE1  -  Gle1p

Saccharomyces cerevisiae S288c

Synonyms: BRR3, D1049, NLE2, Nuclear pore protein GLE1, Nucleoporin GLE1, ...
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High impact information on GLE1

  • In order to identify additional factors required for nuclear export of messenger RNA, a genetic screen was conducted with a yeast mutant deficient in a factor Gle1p, which associates with the nuclear pore complex (NPC) [1].
  • Npl3p, a key mediator of export of poly(A)+ RNA, was not required for heat shock mRNA export, whereas Rss1p/Gle1p, a NES-containing factor essential for poly(A)+ RNA export, was also required for export of heat shock mRNAs after stress [2].
  • Genetic interactions between Rip1p and the RNA export mediator Gle1p are described, which support a role of the carboxyl terminus of Rip1p in poly(A)+ RNA export [3].
  • Dominant mutations in DBP5 and GLE1 that rescue mRNA export phenotypes associated with the lack of InsP6 mimic the InsP6 effects in vitro [4].
  • Interestingly, Gle1 binds directly to inositol hexakisphosphate (InsP6) and InsP6 potentiates the Gle1-mediated stimulation of Dbp5 [4].

Biological context of GLE1


Anatomical context of GLE1


Associations of GLE1 with chemical compounds


Physical interactions of GLE1

  • Dbp5p interacts with Gle1p independently of the N-terminus of Dbp5p [8].
  • Rip1p interacts directly with Gle1p and is the only protein known to be essential for mRNA export after heat shock but not under normal growth conditions [9].

Regulatory relationships of GLE1

  • Overexpression of Dbp5p completely suppressed the growth and mRNA export defects of rat7DeltaN cells and resulted in weaker suppression in cells carrying rat7-1 or the rss1-37 allele of GLE1 [8].

Other interactions of GLE1

  • We show here that overexpression of Rss1/Gle1 also partially rescued depletion of Nup82 [10].
  • Overexpression of Rss1/Gle1, a putative nucleoporin and/or mRNA transport factor, was shown previously to partially rescue depletion of Nup159 [10].
  • Nuclear export of the yeast mRNA-binding protein Nab2 is linked to a direct interaction with Gfd1 and to Gle1 function [11].
  • Gle1, another NPC-associating component, also interacted with Ulp1 in the two-hybrid system and co-immunoprecipitation experiment [12].


  1. A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export. York, J.D., Odom, A.R., Murphy, R., Ives, E.B., Wente, S.R. Science (1999) [Pubmed]
  2. Yeast heat shock mRNAs are exported through a distinct pathway defined by Rip1p. Saavedra, C.A., Hammell, C.M., Heath, C.V., Cole, C.N. Genes Dev. (1997) [Pubmed]
  3. The yeast nucleoporin rip1p contributes to multiple export pathways with no essential role for its FG-repeat region. Stutz, F., Kantor, J., Zhang, D., McCarthy, T., Neville, M., Rosbash, M. Genes Dev. (1997) [Pubmed]
  4. Activation of the DExD/H-box protein Dbp5 by the nuclear-pore protein Gle1 and its coactivator InsP6 is required for mRNA export. Weirich, C.S., Erzberger, J.P., Flick, J.S., Berger, J.M., Thorner, J., Weis, K. Nat. Cell Biol. (2006) [Pubmed]
  5. A Crm1p-independent nuclear export path for the mRNA-associated protein, Npl3p/Mtr13p. Liu, Y., Guo, W., Tartakoff, P.Y., Tartakoff, A.M. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  6. The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr 255p. Strahm, Y., Fahrenkrog, B., Zenklusen, D., Rychner, E., Kantor, J., Rosbach, M., Stutz, F. EMBO J. (1999) [Pubmed]
  7. Cytoplasmic inositol hexakisphosphate production is sufficient for mediating the Gle1-mRNA export pathway. Miller, A.L., Suntharalingam, M., Johnson, S.L., Audhya, A., Emr, S.D., Wente, S.R. J. Biol. Chem. (2004) [Pubmed]
  8. Rat8p/Dbp5p is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpo1-1 cells. Hodge, C.A., Colot, H.V., Stafford, P., Cole, C.N. EMBO J. (1999) [Pubmed]
  9. The nuclear pore complex and the DEAD box protein Rat8p/Dbp5p have nonessential features which appear to facilitate mRNA export following heat shock. Rollenhagen, C., Hodge, C.A., Cole, C.N. Mol. Cell. Biol. (2004) [Pubmed]
  10. Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex. Hurwitz, M.E., Strambio-de-Castillia, C., Blobel, G. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  11. Nuclear export of the yeast mRNA-binding protein Nab2 is linked to a direct interaction with Gfd1 and to Gle1 function. Suntharalingam, M., Alcázar-Román, A.R., Wente, S.R. J. Biol. Chem. (2004) [Pubmed]
  12. Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins. Takahashi, Y., Mizoi, J., Toh-E, A., Kikuchi, Y. J. Biochem. (2000) [Pubmed]
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