High impact information on MRS5

• This interaction of Tim10 and Tim12 with the precursors depends on the presence of divalent metal ions [1].
• Tim10 and Tim12 are found in a complex with Tim22, which takes over the precursor and mediates its membrane-potential-dependent insertion into the inner membrane [1].
• Tim9, an essential protein of Saccharomyces cerevisiae, shares sequence similarity with Tim10 and Tim12 [2].
• Elevated gene dosage of this new gene, termed MRS5, suppresses also the pet phenotype of a mitochondrial splicing-deficient group II intron mutation M1301 [3].
• The MRS5 gene product, a 13-kDa protein of low abundance, shows no similarity to other known proteins and is associated with the inner mitochondrial membrane, protruding into the intermembrane space [3].

Biological context of MRS5

• Interestingly, this new gene shares many similarities with the previously characterized MRS5 gene: when expressed from a multicopy plasmid, MRS11 like MRS5 restores respiration competence to yeast strains defective in the splicing of mitochondrial group II introns [4].
• These findings suggest an essential role of Mrs5p in mitochondrial biogenesis [3].

Anatomical context of MRS5

• Mrs5p, an essential protein of the mitochondrial intermembrane space, affects protein import into yeast mitochondria [3].

Other interactions of MRS5

• Depletion of Mrs5p in yeast cells causes accumulation of unprocessed precursors of the mitochondrial hsp60 protein and defects in all cytochrome complexes [3].

References