High impact information on VPS25

• Intron positions were analyzed in VPS25 from 36 species [1].
• Expression of mammalian VPS25 in a wide range of tissues, and the presence in a broad range of eukaryotic species, indicates a basic role in eukaryotic cell function [1].
• We found evidence for five ancestral VPS25 introns, intron loss, and single instances of intron gain (a Paramecium species) and intron slippage (Theileria species) [1].
• The amino acid sequences of 119 Vps25 orthologs are aligned, compared with the known secondary structure of yeast Vps25p, and used to carry out phylogenetic analysis [1].
• Residues in two amino-terminal PPXY motifs (motif I and II), involved in dimerization of Vps25p and interaction with Vps22p and Vps36p, were closely, but not absolutely conserved [1].

Associations of VPS25 with chemical compounds

• A highly conserved carboxy-terminal lysine was identified, which suggests Vps25 is ubiquitinated [1].

Other interactions of VPS25

• Two PPXY-motifs from Vps25 are involved in contacts with Vps22 and Vps36, and their mutation leads to ESCRT-II disruption [2].
• Vps25 is the chief subunit responsible for Vps20 recruitment [2].

References