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Gene Review

SPT20  -  Spt20p

Saccharomyces cerevisiae S288c

Synonyms: ADA5, Transcription factor SPT20, YOL148C
 
 
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Disease relevance of SPT20

  • In this report we described the cloning and characterization of ADA5, a gene identified by resistance to GAL4-VP16-mediated toxicity [1].
 

High impact information on SPT20

  • We have studied the in vivo role of two other SAGA components, Spt3 and Spt20, at the well-characterized GAL1 promoter [2].
  • Gene induction in response to unfolded protein in the endoplasmic reticulum is mediated through Ire1p kinase interaction with a transcriptional coactivator complex containing Ada5p [3].
  • In addition, partial SAGA complexes containing Spt7 can be assembled in the absence of both Spt20 and Ada1 [4].
  • Further, ADA5 cofractionates and coprecipitates with ADA3 [1].
  • Most interestingly, ADA5 is identical to SPT20 (S.M. Roberts and F. Winston, Mol. Cell. Biol. 16: 3206-3213, 1996), showing that it shares phenotypes with the ADA and SPT family of genes [1].
 

Biological context of SPT20

  • Consistent with its having an important function in transcription in vivo, SPT20 was also recently identified as ADA5 and has been shown to be important for transcriptional activation (G.A. Marcus, J [5].
 

Other interactions of SPT20

  • Systematic analysis has demonstrated that spt20 delta/ada5 delta and spt7 delta mutations cause lethality with every snf/swi and srb/mediator mutation tested [6].
  • Our results demonstrate that Spt3 and Spt20 are required for full delta promoter activity, but that Gcn5, another member of SAGA, is not required [7].
  • The transcriptional co-activator ADA5 is required for HAC1 mRNA processing in vivo [8].
  • Interestingly, the spt3, spt8, and spt20 mutants behaved differently than other Spt-Ada-Gcn5-acetyltransferase (SAGA) mutants [9].

References

  1. ADA5/SPT20 links the ADA and SPT genes, which are involved in yeast transcription. Marcus, G.A., Horiuchi, J., Silverman, N., Guarente, L. Mol. Cell. Biol. (1996) [Pubmed]
  2. The Spt components of SAGA facilitate TBP binding to a promoter at a post-activator-binding step in vivo. Dudley, A.M., Rougeulle, C., Winston, F. Genes Dev. (1999) [Pubmed]
  3. Gene induction in response to unfolded protein in the endoplasmic reticulum is mediated through Ire1p kinase interaction with a transcriptional coactivator complex containing Ada5p. Welihinda, A.A., Tirasophon, W., Green, S.R., Kaufman, R.J. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  4. Analysis of Spt7 function in the Saccharomyces cerevisiae SAGA coactivator complex. Wu, P.Y., Winston, F. Mol. Cell. Biol. (2002) [Pubmed]
  5. SPT20/ADA5 encodes a novel protein functionally related to the TATA-binding protein and important for transcription in Saccharomyces cerevisiae. Roberts, S.M., Winston, F. Mol. Cell. Biol. (1996) [Pubmed]
  6. Essential functional interactions of SAGA, a Saccharomyces cerevisiae complex of Spt, Ada, and Gcn5 proteins, with the Snf/Swi and Srb/mediator complexes. Roberts, S.M., Winston, F. Genetics (1997) [Pubmed]
  7. Specific components of the SAGA complex are required for Gcn4- and Gcr1-mediated activation of the his4-912delta promoter in Saccharomyces cerevisiae. Dudley, A.M., Gansheroff, L.J., Winston, F. Genetics (1999) [Pubmed]
  8. The transcriptional co-activator ADA5 is required for HAC1 mRNA processing in vivo. Welihinda, A.A., Tirasophon, W., Kaufman, R.J. J. Biol. Chem. (2000) [Pubmed]
  9. Screening the yeast "disruptome" for mutants affecting resistance to the immunosuppressive drug, mycophenolic acid. Desmoucelles, C., Pinson, B., Saint-Marc, C., Daignan-Fornier, B. J. Biol. Chem. (2002) [Pubmed]
 
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