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Gene Review:

TOA1 - Toa1p

Saccharomyces cerevisiae S288c

Synonyms: TFIIA 32 kDa subunit, TFIIA large subunit, Transcription initiation factor IIA large subunit, YOR194C

Solow, S.P. et al., Titov, A.A. et al., Liu, Q. et al., Xie, J. et al., Ranish, J.A. et al.

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High impact information on TOA1

Both the TOA1 and TOA2 genes are essential for growth of yeast [1].
The single point mutation in Toa1 alleviates the requirement for both subunits of NC2 [2].
Like other substrate-Kap complexes, the Toa1p/Toa2p/Kap122p complex isolated from yeast cytosol or reconstituted from recombinant proteins, was dissociated by RanGTP but not RanGDP [3].
We found that co-overexpression of TOA1 and TOA2, the genes that encode yeast TFIIA, overcomes the activation defects caused by the TBP mutants [4].
Alanine substitution of serine residues 220, 225, and 232 completely eliminated in vivo phosphorylation of Toa1, although no single amino acid substitution of these serine residues eliminated phosphorylation in vivo [5].

Biological context of TOA1

We report here that Toa1, the large subunit of yeast TFIIA, is phosphorylated in vivo and that this phosphorylation stabilizes the TFIIA-TBP-DNA complex and is required for high-level transcription [5].

Other interactions of TOA1

Deletion of KAP122 is not lethal, although deletion of TOA1 and TOA2 is [3].

References

Isolation of two genes that encode subunits of the yeast transcription factor IIA. Ranish, J.A., Lane, W.S., Hahn, S. Science (1992) [Pubmed]
A single point mutation in TFIIA suppresses NC2 requirement in vivo. Xie, J., Collart, M., Lemaire, M., Stelzer, G., Meisterernst, M. EMBO J. (2000) [Pubmed]
The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus. Titov, A.A., Blobel, G. J. Cell Biol. (1999) [Pubmed]
Analysis of TFIIA function In vivo: evidence for a role in TATA-binding protein recruitment and gene-specific activation. Liu, Q., Gabriel, S.E., Roinick, K.L., Ward, R.D., Arndt, K.M. Mol. Cell. Biol. (1999) [Pubmed]
Phosphorylation of TFIIA stimulates TATA binding protein-TATA interaction and contributes to maximal transcription and viability in yeast. Solow, S.P., Lezina, L., Lieberman, P.M. Mol. Cell. Biol. (1999) [Pubmed]

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AGOS_AGR228C	Ashbya gossypii ATCC 10895
KLLA0F00748g	Kluyveromyces lactis NRRL Y-1140

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