High impact information on WTM1

• Forced localization of Wtm1 to the nucleolus causes Rnr2/Rnr4 complexes to relocalize to the nucleolus [1].
• Wtm1 binds Rnr2/4 complexes and release them to the cytoplasm in response to DNA damage [1].
• Deletion of either WTM1 or KAP122 leads to loss of betabeta' nuclear localization [2].
• These data suggested that Wtm1p and Wtm2p are repressors and that Wtm3p has different effects on transcription at different loci [3].
• Further investigation reveals that Wtm2p and Wtm1p, previously described as regulators of meiotic gene expression and transcriptional silencing, amplify transcriptional induction of RNR3 in response to replication stress and modulate expression of genes encoding other RNR subunits [4].

Biological context of WTM1

• From this screen we isolated a plasmid encoding two closely related paralogs, WTM1 and WTM2, that greatly increases constitutive expression of RNR3-lacZ [4].
• Thus, Wtm1 acts as a nuclear anchor to maintain nuclear localization of Rnr2/4 complexes outside of S phase [1].
• Our results suggest that Wtm1 acts either as an adaptor to facilitate nuclear import of betabeta' by Kap122 or as an anchor to retain betabeta' in the nucleus [2].

References