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Gene Review:

MET28 - Met28p

Saccharomyces cerevisiae S288c

Synonyms: Methionine-requiring protein 28, Transcriptional activator of sulfur metabolism MET28, YIR017C

Thomas, D. et al., Kuras, L. et al., Kuras, L. et al., Blaiseau, P.L. et al., Aburatani, S. et al.

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High impact information on MET28

Situated upstream of the sulfur genes, this element is the binding site of a transcription activation complex consisting of a basic helix-loop-helix factor, Cbf1p, and two basic leucine zipper factors, Met4p and Met28p [1].
Molecular studies have unraveled the specific functions for each subunit of the Cbf1p-Met4p-Met28p complex as well as the modalities of its assembly on the DNA [1].
We also demonstrate that Met28 possesses no intrinsic transcription activation capabilities [2].
Our results give evidence that the leucine zippers of Met4 and Met28, along with the basic helix-loop-helix domain of Cbf1, provide the protein surfaces mediating these interactions [2].
Here we report the downstream regulatory subnetworks for UME6 and MET28 [3].

Biological context of MET28

Cbf1 belongs to the basic helix-loop-helix DNA-binding protein family while Met4 and Met28 are two basic leucine zipper (bZIP) factors [4].

Physical interactions of MET28

Moreover, Met28 is shown to enhance the DNA-binding activity of Cbf1 [4].

Other interactions of MET28

In this paper, we demonstrate that other pathways are used to recruit Met4p on the 5' upstream region of the two genes, MET3 and MET28 [5].

Analytical, diagnostic and therapeutic context of MET28

By using mobility shift assays, we report here that the in vitro reconstitution of the Cbf1-Met4-Met28 complex on MET16UAS can be obtained with purified recombinant proteins [4].

References

Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Thomas, D., Surdin-Kerjan, Y. Microbiol. Mol. Biol. Rev. (1997) [Pubmed]
A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism. Kuras, L., Cherest, H., Surdin-Kerjan, Y., Thomas, D. EMBO J. (1996) [Pubmed]
Discovery of novel transcription control relationships with gene regulatory networks generated from multiple-disruption full genome expression libraries. Aburatani, S., Tashiro, K., Savoie, C.J., Nishizawa, M., Hayashi, K., Ito, Y., Muta, S., Yamamoto, K., Ogawa, M., Enomoto, A., Masaki, M., Watanabe, S., Maki, Y., Takahashi, Y., Eguchi, Y., Sakaki, Y., Kuhara, S. DNA Res. (2003) [Pubmed]
Assembly of a bZIP-bHLH transcription activation complex: formation of the yeast Cbf1-Met4-Met28 complex is regulated through Met28 stimulation of Cbf1 DNA binding. Kuras, L., Barbey, R., Thomas, D. EMBO J. (1997) [Pubmed]
Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA. Blaiseau, P.L., Thomas, D. EMBO J. (1998) [Pubmed]

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