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Gene Review

CBF1  -  Cbf1p

Saccharomyces cerevisiae S288c

Synonyms: CBP-1, CEP1, CP1, CPF1, Centromere promoter factor 1, ...
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Disease relevance of CBF1


Psychiatry related information on CBF1


High impact information on CBF1


Chemical compound and disease context of CBF1


Biological context of CBF1


Anatomical context of CBF1


Associations of CBF1 with chemical compounds

  • However, we show that induction of the sulfate assimilation pathway is not achieved solely by CBF1 [10].
  • The enhanced transcription was dependent on the CBF1 gene, but did not compete with an excess of wild-type Met4p, suggesting that some changes in the affinity of Met4p to other factors might be involved in S-adenosylmethionine-mediated transcriptional regulation [15].
  • Role of the Saccharomyces cerevisiae general regulatory factor CP1 in methionine biosynthetic gene transcription [11].
  • C. glabrata cells containing the CBF1 gene under the influence of a shutdown promoter (tetO-ScHOP) arrested their growth after 5 h of cultivation in the presence of the reactive drug doxycycline [16].
  • We find that CBF3 is absolutely essential for this attachment but, contrary to previous reports (Hyman, A. A., K. Middleton, M. Centola, T.J. Mitchison, and J. Carbon. 1992. Microtubule-motor activity of a yeast centromere-binding protein complex. Nature (Lond.). 359:533-536) is not sufficient [9].

Physical interactions of CBF1

  • Cbf1p is a sequence specific DNA binding protein required for MET16 chromatin remodelling and transcription [17].
  • In addition, the CBF1 transcriptional activator can interact with the ARABIDOPSIS GCN5 and ADA2 proteins [18].
  • Moreover, Met28 is shown to enhance the DNA-binding activity of Cbf1 [19].
  • The multifunctional DNA-binding proteins ABF1 and CPF1 bind in a mutually exclusive manner to the promoter region of the QCR8 gene, which encodes 11-kDa subunit VIII of the Saccharomyces cerevisiae mitochondrial ubiquinol-cytochrome c oxidoreductase (QCR) [20].
  • Cbf1p has been shown to interact with the chromatin-remodeling ATPase Isw1p [21].

Regulatory relationships of CBF1

  • Functional analyses after the separate introduction of point mutations into both elements reveal no role for the latter protein and only a minor role for Cpf1p in the regulated expression of CYT1/lacZ chimaeric proteins [22].
  • Physical and functional interactions of Arabidopsis ADA2 transcriptional coactivator proteins with the acetyltransferase GCN5 and with the cold-induced transcription factor CBF1 [23].
  • Detailed analyses of the LAC1 promoter demonstrated that transcription of this gene is inhibited by the presence of the transcription factor Cbf1p and the anaerobic repressor Rox1p [24].

Other interactions of CBF1

  • MET16-CYC1-lacZ reporter constructs were used to show that MET16 5'-flanking DNA contains a CP1-dependent upstream activation sequence (UAS) [11].
  • Synthetic lethal interactions between a cep1 null mutation and mutations in either NDC10 or CEP3 were also detected [12].
  • The requirement for Met4 in regulating GSH1 expression is lost in the absence of the centromere-binding protein Cbf1 [25].
  • The suppressing plasmids contained either CEP1 or DNA derived from the PHO4 locus [26].
  • Spontaneously arising extragenic suppressors of cep1 methionine auxotrophy were also isolated; approximately one-third of them were alleles of pho80 [26].

Analytical, diagnostic and therapeutic context of CBF1


  1. Yeast centromere binding protein CBF1, of the helix-loop-helix protein family, is required for chromosome stability and methionine prototrophy. Cai, M., Davis, R.W. Cell (1990) [Pubmed]
  2. Isolation of the gene encoding the Saccharomyces cerevisiae centromere-binding protein CP1. Baker, R.E., Masison, D.C. Mol. Cell. Biol. (1990) [Pubmed]
  3. Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor. Bram, R.J., Kornberg, R.D. Mol. Cell. Biol. (1987) [Pubmed]
  4. Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore. Jiang, W., Lechner, J., Carbon, J. J. Cell Biol. (1993) [Pubmed]
  5. Identification of cohesin association sites at centromeres and along chromosome arms. Tanaka, T., Cosma, M.P., Wirth, K., Nasmyth, K. Cell (1999) [Pubmed]
  6. Budding yeast centromere composition and assembly as revealed by in vivo cross-linking. Meluh, P.B., Koshland, D. Genes Dev. (1997) [Pubmed]
  7. Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Thomas, D., Surdin-Kerjan, Y. Microbiol. Mol. Biol. Rev. (1997) [Pubmed]
  8. Point mutations that separate the role of Saccharomyces cerevisiae centromere binding factor 1 in chromosome segregation from its role in transcriptional activation. Foreman, P.K., Davis, R.W. Genetics (1993) [Pubmed]
  9. Factors required for the binding of reassembled yeast kinetochores to microtubules in vitro. Sorger, P.K., Severin, F.F., Hyman, A.A. J. Cell Biol. (1994) [Pubmed]
  10. MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae. Thomas, D., Jacquemin, I., Surdin-Kerjan, Y. Mol. Cell. Biol. (1992) [Pubmed]
  11. Role of the Saccharomyces cerevisiae general regulatory factor CP1 in methionine biosynthetic gene transcription. O'Connell, K.F., Surdin-Kerjan, Y., Baker, R.E. Mol. Cell. Biol. (1995) [Pubmed]
  12. Evidence that the MIF2 gene of Saccharomyces cerevisiae encodes a centromere protein with homology to the mammalian centromere protein CENP-C. Meluh, P.B., Koshland, D. Mol. Biol. Cell (1995) [Pubmed]
  13. CBP1 associates with the Dictyostelium cytoskeleton and is important for normal cell aggregation under certain developmental conditions. Dharamsi, A., Tessarolo, D., Coukell, B., Pun, J. Exp. Cell Res. (2000) [Pubmed]
  14. In vivo analysis of sequences necessary for CBP1-dependent accumulation of cytochrome b transcripts in yeast mitochondria. Mittelmeier, T.M., Dieckmann, C.L. Mol. Cell. Biol. (1993) [Pubmed]
  15. Single point mutations in Met4p impair the transcriptional repression of MET genes in Saccharomyces cerevisiae. Omura, F., Fujita, A., Shibano, Y. FEBS Lett. (1996) [Pubmed]
  16. Multifunctional centromere binding factor 1 is essential for chromosome segregation in the human pathogenic yeast Candida glabrata. Stoyan, T., Gloeckner, G., Diekmann, S., Carbon, J. Mol. Cell. Biol. (2001) [Pubmed]
  17. Histone acetylation, chromatin remodelling, transcription and nucleotide excision repair in S. cerevisiae: studies with two model genes. Teng, Y., Yu, Y., Ferreiro, J.A., Waters, R. DNA Repair (Amst.) (2005) [Pubmed]
  18. Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression. Stockinger, E.J., Mao, Y., Regier, M.K., Triezenberg, S.J., Thomashow, M.F. Nucleic Acids Res. (2001) [Pubmed]
  19. Assembly of a bZIP-bHLH transcription activation complex: formation of the yeast Cbf1-Met4-Met28 complex is regulated through Met28 stimulation of Cbf1 DNA binding. Kuras, L., Barbey, R., Thomas, D. EMBO J. (1997) [Pubmed]
  20. Global regulation of mitochondrial biogenesis in Saccharomyces cerevisiae: ABF1 and CPF1 play opposite roles in regulating expression of the QCR8 gene, which encodes subunit VIII of the mitochondrial ubiquinol-cytochrome c oxidoreductase. de Winde, J.H., Grivell, L.A. Mol. Cell. Biol. (1992) [Pubmed]
  21. Cbf1p is required for chromatin remodeling at promoter-proximal CACGTG motifs in yeast. Kent, N.A., Eibert, S.M., Mellor, J. J. Biol. Chem. (2004) [Pubmed]
  22. Interactions of the yeast centromere and promoter factor, Cpf1p, with the cytochrome c1 upstream region and functional implications on regulated gene expression. Oechsner, U., Bandlow, W. Nucleic Acids Res. (1996) [Pubmed]
  23. Physical and functional interactions of Arabidopsis ADA2 transcriptional coactivator proteins with the acetyltransferase GCN5 and with the cold-induced transcription factor CBF1. Mao, Y., Pavangadkar, K.A., Thomashow, M.F., Triezenberg, S.J. Biochim. Biophys. Acta (2006) [Pubmed]
  24. Differential regulation of ceramide synthase components LAC1 and LAG1 in Saccharomyces cerevisiae. Kolaczkowski, M., Kolaczkowska, A., Gaigg, B., Schneiter, R., Moye-Rowley, W.S. Eukaryotic Cell (2004) [Pubmed]
  25. Coupling of the transcriptional regulation of glutathione biosynthesis to the availability of glutathione and methionine via the Met4 and Yap1 transcription factors. Wheeler, G.L., Trotter, E.W., Dawes, I.W., Grant, C.M. J. Biol. Chem. (2003) [Pubmed]
  26. Possible cross-regulation of phosphate and sulfate metabolism in Saccharomyces cerevisiae. O'Connell, K.F., Baker, R.E. Genetics (1992) [Pubmed]
  27. Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae. Wieland, G., Hemmerich, P., Koch, M., Stoyan, T., Hegemann, J., Diekmann, S. Nucleic Acids Res. (2001) [Pubmed]
  28. Nuclear localization of CBF1 is regulated by interactions with the SMRT corepressor complex. Zhou, S., Hayward, S.D. Mol. Cell. Biol. (2001) [Pubmed]
  29. Generation of temperature-sensitive cbp1 strains of Saccharomyces cerevisiae by PCR mutagenesis and in vivo recombination: characteristics of the mutant strains imply that CBP1 is involved in stabilization and processing of cytochrome b pre-mRNA. Staples, R.R., Dieckmann, C.L. Genetics (1993) [Pubmed]
  30. CBP1 function is required for stability of a hybrid cob-oli1 transcript in yeast mitochondria. Mittelmeier, T.M., Dieckmann, C.L. Curr. Genet. (1990) [Pubmed]
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