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MeSH Review

Electrophoretic Mobility Shift Assay

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Disease relevance of Electrophoretic Mobility Shift Assay


Psychiatry related information on Electrophoretic Mobility Shift Assay

  • To elucidate the neurochemical mechanism that underlies the effect of anti-parkinsonian agents on motor activities in the dopamine-depleted striatum, we evaluated AP-I and CREB DNA-binding activity in the striatum of 6-hydroxydopamine-lesioned rats by use of a gel mobility-shift assay [6].

High impact information on Electrophoretic Mobility Shift Assay

  • Electrophoretic mobility shift assays and reporter assays showed that Cbfbeta was necessary for the efficient DNA binding of Runx2 and for Runx2-dependent transcriptional activation [7].
  • Evidence of LMP1-TRAF signaling was sought with an electrophoretic mobility shift assay for the nuclear factor-kappaB (NF-kappaB) transcription factor [8].
  • An electrophoretic mobility shift assay revealed activated NF-kappaB in all eight EBV-positive, LMP1-positive samples as well, but not in either of the EBV-negative, LMP1-negative samples or in the three EBV-positive, LMP1-negative samples [8].
  • Gel retardation assays showed that p53 formed highly stable complexes when the DNA contained the IDL mismatches, but only unstable complexes when the DNA lacked lesions (but did contain free ends) [9].
  • Second, mobility-shift assays of muscle cell nuclear extracts, "double shifted" with specific antisera, have identified complexes binding to the MEF1 site that contain either MyoD or myogenin in association with E12/E47-like proteins [10].

Chemical compound and disease context of Electrophoretic Mobility Shift Assay


Biological context of Electrophoretic Mobility Shift Assay


Anatomical context of Electrophoretic Mobility Shift Assay


Associations of Electrophoretic Mobility Shift Assay with chemical compounds


Gene context of Electrophoretic Mobility Shift Assay


Analytical, diagnostic and therapeutic context of Electrophoretic Mobility Shift Assay


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