Disease relevance of MET3

• Production, purification, and luminometric analysis of recombinant Saccharomyces cerevisiae MET3 adenosine triphosphate sulfurylase expressed in Escherichia coli [1].
• The synthesis of ATP sulfurylase was directed by an expression system that employs the regulatory genes of the luminous bacterium Vibrio fischeri [1].
• Crystal structure of a novel zinc-binding ATP sulfurylase from Thermus thermophilus HB8 [2].

High impact information on MET3

• Crystal structure of ATP sulfurylase from Saccharomyces cerevisiae, a key enzyme in sulfate activation [3].
• Recent developments have provided new insight into the nature and control of the enzymes ATP sulfurylase and APS kinase, which catalyze the early steps of sulfate assimilation, and of the Aspergillus enzyme, cysteine synthase, which produces cysteine from O-acetylserine [4].
• Heterologous complementation restores only ATP sulfurylase function as demonstrated by low [35S]sulfate influx measurements and selenate resistance [5].
• ASA1 is specified by a single copy gene that is part of a multigene family in A. thaliana [5].
• Regulation of inorganic sulfate activation in filamentous fungi. Allosteric inhibition of ATP sulfurylase by 3'-phosphoadenosine-5'-phosphosulfate [6].

Biological context of MET3

• A conditional strain expressing OLE1 from the regulatable MET3 promoter was unable to grow in repressing conditions, indicating that OLE1 is an essential gene [7].
• A regulated MET3-GLC7 gene fusion provides evidence of a mitotic role for Saccharomyces cerevisiae protein phosphatase 1 [8].
• The MET3 gene of Saccharomyces cerevisiae was cloned and its restriction map was found to differ in the upstream region from an earlier published map (Cherest et al. Gene 34, 269-281, 1985) and nucleotide sequence (Cherest et al. Mol. Gen. Genet. 210, 307-313, 1987) [9].
• TDH2 is linked to MET3 on chromosome X of Saccharomyces cerevisiae [9].
• Plasmids bearing different deletions upstream of the transcribed region of MET3 were constructed [10].

Anatomical context of MET3

• In Arabidopsis thaliana cDNA cloning revealed the existence of three ATP sulfurylase isoforms (APS1, -2, and -3) all of which appear to be localized in plastids [11].
• ATP sulfurylase (ATP: sulfate adenylyl transferase, EC 2.7.7.4), the first enzyme of the sulfate assimilation pathway, is present in the chloroplast and cytosol of plants [11].

Associations of MET3 with chemical compounds

• The beta-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium [12].
• A number of genes have been implicated in the regulation of laccase and melanization, including IPC1, GPA1, MET3, and STE12 [13].
• Partial repression of MET3p-OLE1 slightly lowered oleic acid levels and decreased membrane fluidity; these conditions permitted growth in the yeast form, but prevented hyphal development in aerobic conditions and blocked the formation of chlamydospores [7].
• Sulfate assimilation in Aspergillus terreus: analysis of genes encoding ATP-sulfurylase and PAPS-reductase [14].
• We used URA3 as a model to demonstrate that the MET3 promoter could control the expression of an essential gene, provided that a mixture of both methionine and cysteine was used to repress the promoter [15].

Regulatory relationships of MET3

• Over-expression of MET4 resulted in leaky expression from the otherwise tightly regulated MET3 promoter under its control [16].
• The genes encoding GDP-mannose pyrophosphorylase from Saccharomyces cerevisiae (SRB1/PSA1) and Candida albicans (CaSRB1) were expressed under the control of the tightly regulated promoters of MET3 and CaMET3 respectively [17].

Other interactions of MET3

• In contrast, transcription of the MET3-URA3 fusion did not alter the Ty1 target-site distribution in wild-type or other mutant strains [18].
• Three genes, MET3, MET14 and MET16, are essential for this reduction [19].
• Both genes are similar to their homologs in A. nidulans (sA and sC), Penicillium chrysogenum (aps) and Saccharomyces cerevisiae (MET3 and MET16) [14].
• To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter [12].
• We have sequenced a 42,500 bp stretch located on chromosome X of Saccharomyces cerevisiae between the genes MET3 and CDC8 [20].

Analytical, diagnostic and therapeutic context of MET3

• Based on sequence analysis, it is hypothesized that APS2 may encode the cytosolic ATP sulfurylase [11].
• Analysis of genomic DNA by Southern blotting revealed that multiple genes encode ATP sulfurylase in soybean [21].
• Northern blot analysis indicated a decline in the ATP sulfurylase transcript levels during seed development [21].

References