Disease relevance of SPC98

• The toxicity of SPC98 overexpression was relieved by co-overexpression of TUB4 [1].

High impact information on SPC98

• The yeast Xgrip109 homologue, Spc98, is a spindle-pole body component that interacts with gamma tubulin [2].
• Primers derived from the human expressed sequence tag matching SPC98 were used to clone the 5' and 3' cDNA ends by rapid amplification of cDNA ends (RACE)-PCR [3].
• The human Spc98 cDNA presents an alternative splicing at the 3' end [3].
• Strong overexpression of SPC98 is toxic, causing cells to arrest with a large bud, defective microtubule structures, undivided nucleus and replicated DNA [1].

Biological context of SPC98

• Evidence is provided for a physical and functional interaction between Tub4p, Spc98p and Spc97p: first, temperature-sensitive spc97(ts) mutants are suppressed by high gene dosage of SPC98 or TUB4 [4].

Anatomical context of SPC98

• Previously, we have shown that the gamma-tubulin Tub4p and the spindle pole body component Spc98p are involved in microtubule organization by the yeast microtubule organizing centre, the spindle pole body (SPB) [4].

Physical interactions of SPC98

• The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment [1].

Other interactions of SPC98

• The screen identified mutations in SPC97 and SPC98, two genes encoding components of the Tub4p complex in yeast [5].

References