The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

PRPF18  -  pre-mRNA processing factor 18

Homo sapiens

Synonyms: HPRP18, PRP18, PRP18 homolog, Pre-mRNA-splicing factor 18, hPRP18, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on PRPF18

  • Splicing activity can be restored to hPrp18-depleted HeLa cell extracts by yeast Prp18, showing that important functional regions of the proteins have been conserved [1].
  • The hPrp18 protein is bound tightly to the spliceosome only during the second step of splicing. hPrp18 is required for the splicing of several pre-mRNAs, making it the first general second-step splicing factor found in humans [1].
  • A 55-kD protein that cross-reacts with antibodies against hPrp18 is a constituent of the U4/U6 and U4/U6 x U5 snRNP particles [1].
  • USA-CyP forms separate, stable complexes with hPrp18 and hPrp4 in which the active site of the cyclophilin is exposed [2].
  • The cyclophilin inhibitor cyclosporin A slows pre-mRNA splicing in vitro, and we show that its inhibition of the second step of splicing is caused by blocking the action of USA-CyP within its complex with hPrp18 [2].

Biological context of PRPF18

  • The spliceosomal cyclophilin H is a specific component of the human U4/U6 small nuclear ribonucleoprotein particle, interacting with homologous sequences in the proteins U4/U6-60K and hPrp18 during pre-mRNA splicing [3].

Anatomical context of PRPF18

  • In HeLa cell extracts immunodepleted of hPrp18, the second step of pre-mRNA splicing is abolished [1].


  1. A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor. Horowitz, D.S., Krainer, A.R. Genes Dev. (1997) [Pubmed]
  2. A cyclophilin functions in pre-mRNA splicing. Horowitz, D.S., Lee, E.J., Mabon, S.A., Misteli, T. EMBO J. (2002) [Pubmed]
  3. Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide. Reidt, U., Wahl, M.C., Fasshauer, D., Horowitz, D.S., Lührmann, R., Ficner, R. J. Mol. Biol. (2003) [Pubmed]
WikiGenes - Universities