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Gene Review

PRPF4  -  pre-mRNA processing factor 4

Homo sapiens

Synonyms: HPRP4, HPRP4P, PRP4, PRP4 homolog, Prp4p, ...
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High impact information on PRPF4


Biological context of PRPF4

  • Mapping of the human HPRP3 and HPRP4 genes encoding U4/U6-associated splicing factors to chromosomes 1q21.1 and 9q31-q33 [5].
  • A mutational analysis of prp4 revealed that the protein consists of a short N-terminal domain, containing several essential motifs, which is followed by the kinase catalytic domain comprising the C-terminus of the protein [6].
  • Overexpression of N-terminal mutations disturbs mitosis and produces elongated cells, Using a PCR approach, we isolated a putative homologue of Prp4 from human and mouse cells [6].

Anatomical context of PRPF4

  • Our results lead to a model in which USA-CyP is carried into the spliceosome in complexes with hPrp4 and hPrp18, and USA-CyP acts during splicing within these complexes [1].

Associations of PRPF4 with chemical compounds

  • Amino acid analysis of intact PRP-6 and sequence determination of PRP-6 chymotryptic peptides, residues 15-24 and 26-35, show a single difference in PRP-6, compared to the most similar, characterized PRP, PRP-4, in that residue 30 is histidine in PRP-6, rather than arginine as in PRP-4 and in all the other sequenced PRPs [7].

Other interactions of PRPF4

  • A database homology search revealed a significant degree of overall homology (57.8% similarity, 33.9% identity) between the human 60kD protein and the Saccharomyces cerevisiae U4/U6 snRNP protein Prp4p [8].
  • A faster adsorption process is observed for PRP-3, PRP-4, PIF-f, and statherin [9].

Analytical, diagnostic and therapeutic context of PRPF4

  • Northern blot analysis revealed that hPRP4 mRNA was ubiquitously expressed in multiple tissues [4].
  • Indirect immunofluorescence analysis revealed that endogenous hPRP4 was distributed in a nuclear speckled pattern and colocalized with SF2/ASF in HeLa S3 cells [4].


  1. A cyclophilin functions in pre-mRNA splicing. Horowitz, D.S., Lee, E.J., Mabon, S.A., Misteli, T. EMBO J. (2002) [Pubmed]
  2. Mammalian PRP4 kinase copurifies and interacts with components of both the U5 snRNP and the N-CoR deacetylase complexes. Dellaire, G., Makarov, E.M., Cowger, J.J., Longman, D., Sutherland, H.G., Lührmann, R., Torchia, J., Bickmore, W.A. Mol. Cell. Biol. (2002) [Pubmed]
  3. Identification and characterization of human genes encoding Hprp3p and Hprp4p, interacting components of the spliceosome. Wang, A., Forman-Kay, J., Luo, Y., Luo, M., Chow, Y.H., Plumb, J., Friesen, J.D., Tsui, L.C., Heng, H.H., Woolford, J.L., Hu, J. Hum. Mol. Genet. (1997) [Pubmed]
  4. Cloning of human PRP4 reveals interaction with Clk1. Kojima, T., Zama, T., Wada, K., Onogi, H., Hagiwara, M. J. Biol. Chem. (2001) [Pubmed]
  5. Mapping of the human HPRP3 and HPRP4 genes encoding U4/U6-associated splicing factors to chromosomes 1q21.1 and 9q31-q33. Heng, H.H., Wang, A., Hu, J. Genomics (1998) [Pubmed]
  6. Functional analysis of the fission yeast Prp4 protein kinase involved in pre-mRNA splicing and isolation of a putative mammalian homologue. Gross, T., Lützelberger, M., Weigmann, H., Klingenhoff, A., Shenoy, S., Käufer, N.F. Nucleic Acids Res. (1997) [Pubmed]
  7. Primary structure of a novel human salivary acidic proline-rich protein. Schlesinger, D.H., Hay, D.I., Schluckebier, S.K., Ahern, J.M. Pept. Res. (1994) [Pubmed]
  8. The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p. Lauber, J., Plessel, G., Prehn, S., Will, C.L., Fabrizio, P., Gröning, K., Lane, W.S., Lührmann, R. RNA (1997) [Pubmed]
  9. Temporal and compositional characteristics of salivary protein adsorption to hydroxyapatite. Lamkin, M.S., Arancillo, A.A., Oppenheim, F.G. J. Dent. Res. (1996) [Pubmed]
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