Disease relevance of SSE1

• Similarly, deletion of SSE1 causes a firefly luciferase folding defect in yeast cells under heat stress in vivo [1].

High impact information on SSE1

• On the other hand, the absence of SSE1 enhances polypeptide binding to both SSB and SSA and impairs cell growth [2].
• To test the in vivo requirement for interdomain communication, SSE1 alleles carrying amino acid substitutions in the ATPase domain were assayed for their ability to complement sse1Delta yeast [3].
• To elucidate the mechanism of Ydj1p action, and to identify functional partners, we screened for multicopy suppressors of the temperature-sensitive ydj1-151 mutant and identified a yeast Hsp110, SSE1 [4].
• Heat shock induction of SSE1, encoding a member of the Hsp110 family of heat shock proteins, was also dependent on the HSF CTA [5].
• In this report, we demonstrate a novel synthetic genetic interaction between SSE1 and SCH9 [6].

Biological context of SSE1

• Isolation and characterization of SSE1 and SSE2, new members of the yeast HSP70 multigene family [7].
• Disruption of the MSI3 gene was associated with a temperature sensitive growth phenotype but unexpectedly, thermotolerance was enhanced in the disruptant [8].
• The consensus nucleotide sequence of the heat shock element (HSE) was found in the upstream region of MSI3 [8].
• The amino acid sequence of Msi3p is about 30% identical to that of the Ssa1p of Saccharomyces cerevisiae [8].

Regulatory relationships of SSE1

• Overexpression of MSI3 also suppresses the heat shock-sensitive phenotype of the bcy1 mutant [8].

Other interactions of SSE1

• SSE1 and SSE2 are thought to encode polypeptides of 693 aa with calculated M(r)'s of 77,408 and 77,619, respectively [7].
• The MSI3 gene was isolated as a multicopy suppressor of the heat shock-sensitive phenotype of the ira1 mutation, which causes hyperactivation of the RAS-cAMP pathway [8].

References