Disease relevance of LEU5

• Here, we investigated the yeast carrier Leu5p (encoded by the gene YHR002w) and its close human homologue Graves' disease protein [1].

High impact information on LEU5

• Deletion of LEU5 (strain Deltaleu5) was accompanied by a 15-fold reduction of mitochondrial coenzyme A (CoA) levels but did not affect the cytosolic CoA content [1].
• The yeast mitochondrial carrier Leu5p and its human homologue Graves' disease protein are required for accumulation of coenzyme A in the matrix [1].
• Our in vitro and in vivo analyses assign a function to Leu5p in the accumulation of CoA in mitochondria, presumably by serving as a transporter of CoA or a precursor thereof [1].
• This shows that LEU5 is not essential for leucine biosynthesis [2].
• LEU5 maps to chromosome VIII and is tightly linked to CEN8 [2].

Biological context of LEU5

• Five different mutations in LEU5 were analyzed: a site-directed deletion and a disruption, as well as three distinct mutations produced by chemical mutagenesis [2].
• In a leu4 background, each leu5 mutation causes a Leu--phenotype; in a LEU4 background, none of the mutations alters the Leu+ phenotype [2].

Other interactions of LEU5

• In either a leu4 or LEU4 background, each leu5 mutation causes a glycerol--phenotype [2].

References