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Gene Review:

DED81 - asparagine--tRNA ligase DED81

Saccharomyces cerevisiae S288c

Synonyms: AsnRS, Asparagine--tRNA ligase, cytoplasmic, Asparaginyl-tRNA synthetase, YHR019C

Landrieu, I. et al., Schultes, N.P. et al., Adjiri, A. et al.

Landrieu, Vandenbol, Leberman, Portetelle, Hartlein,

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Disease relevance of DED81

Expression of YHR019 in Escherichia coli demonstrated that a yeast AsnRS activity was produced [1].

High impact information on DED81

We have constructed eight restriction site polymorphisms in the DED81-ARG4 region and examined their behavior during meiotic recombination [2].
There is a second gradient of gene conversion in DED81, with high levels near the 5' end of the gene and low levels near the middle of the gene [2].
The isolated gene could be identified to the open reading frame YHR019, called DED81, located on chromosome VIII [1].
We showed that the recombinant S. cerevisiae AsnRS was able to charge unfractionated yeast tRNA, but not E. coli tRNA, with asparagine [1].

Biological context of DED81

It contains besides the ARG4 gene, another complete open reading frame (ORF) (YSD83) and a third incomplete one (DED81) [3].

Associations of DED81 with chemical compounds

Exploiting the asparagine auxotrophy of the Saccharomyces cerevisiae mutant strain 8556a, we have isolated the gene for the cytosolic asparaginyl-tRNA synthetase (AsnRS) of S. cerevisiae, by functional complementation of the mutation affecting this strain [1].

References

Identification of YHR019 in Saccharomyces cerevisiae chromosome VIII as the gene for the cytosolic asparaginyl-tRNA synthetase. Landrieu, I., Vandenbol, M., Leberman, R., Portetelle, D., Hartlein, M. Yeast (1998) [Pubmed]
Decreasing gradients of gene conversion on both sides of the initiation site for meiotic recombination at the ARG4 locus in yeast. Schultes, N.P., Szostak, J.W. Genetics (1990) [Pubmed]
Sequence comparison of the ARG4 chromosomal regions from the two related yeasts, Saccharomyces cerevisiae and Saccharomyces douglasii. Adjiri, A., Chanet, R., Mezard, C., Fabre, F. Yeast (1994) [Pubmed]

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