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Gene Review

SIT1  -  Sit1p

Saccharomyces cerevisiae S288c

Synonyms: ARN3, Ferrioxamine B permease, Siderophore iron transporter 1, Siderophore iron transporter ARN3, YEL065W
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High impact information on SIT1

  • Deletion of ARN3 did not prevent yeast from using ferrioxamine B as an iron source; however, deletion of ARN3 and FET3, a component of the high affinity ferrous iron transport system, did prevent uptake of ferrioxamine-bound iron and growth on ferrioxamine as an iron source [1].
  • One of these, Arn3p/Sit1p, facilitates the uptake of ferrioxamine B, a siderophore of the hydroxamate class [2].
  • A mutation of FTR1 did not impair the use of these siderophores but did affect the uptake of ferrioxamines E and B, as well as of ferric citrate, indicating that their utilization was independent of Sit1p [3].
  • The human fungal pathogen Candida albicans contains a close homologue of yeast siderophore transporters, designated Sit1p/Arn1p [3].
  • Heterologous expression of SIT1 in the yeast Saccharomyces cerevisiae confirmed the function of Sit1p as a transporter for ferrichrome-type siderophores [3].

Biological context of SIT1

  • From yeast genome sequencing data six genes of the unknown major facilitator (UMF) family were known of which YEL065w Sce was recently identified as a transporter for the bacterial siderophore ferrioxamine B (Sit1p) [4].

Anatomical context of SIT1


Associations of SIT1 with chemical compounds

  • Genes encoding transporters for heterologous siderophores have been identified in Saccharomyces cerevisiae, of which SIT1, TAF1, and ENB1 encode the transporters for ferrioxamines, ferric triacetylfusarinine C and ferric enterobactin, respectively [5].

Other interactions of SIT1

  • These results suggest that siderophore uptake by Sit1p/Arn1p is required in a specific process of C. albicans infection, namely epithelial invasion and penetration, while in the blood or within organs other sources of iron, including heme, may be used [3].
  • For these experiments, we utilized yeast lacking the major iron uptake pathways (FET3 and FET4) and yeast deficient in SIT1, encoding the major up-regulated iron siderophore transporter [6].


  1. Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake. Yun, C.W., Ferea, T., Rashford, J., Ardon, O., Brown, P.O., Botstein, D., Kaplan, J., Philpott, C.C. J. Biol. Chem. (2000) [Pubmed]
  2. Siderophore-iron uptake in saccharomyces cerevisiae. Identification of ferrichrome and fusarinine transporters. Yun, C.W., Tiedeman, J.S., Moore, R.E., Philpott, C.C. J. Biol. Chem. (2000) [Pubmed]
  3. The siderophore iron transporter of Candida albicans (Sit1p/Arn1p) mediates uptake of ferrichrome-type siderophores and is required for epithelial invasion. Heymann, P., Gerads, M., Schaller, M., Dromer, F., Winkelmann, G., Ernst, J.F. Infect. Immun. (2002) [Pubmed]
  4. Identification of a fungal triacetylfusarinine C siderophore transport gene (TAF1) in Saccharomyces cerevisiae as a member of the major facilitator superfamily. Heymann, P., Ernst, J.F., Winkelmann, G. Biometals (1999) [Pubmed]
  5. Identification and substrate specificity of a ferrichrome-type siderophore transporter (Arn1p) in Saccharomyces cerevisiae. Heymann, P., Ernst, J.F., Winkelmann, G. FEMS Microbiol. Lett. (2000) [Pubmed]
  6. Relationship between chloroquine toxicity and iron acquisition in Saccharomyces cerevisiae. Emerson, L.R., Nau, M.E., Martin, R.K., Kyle, D.E., Vahey, M., Wirth, D.F. Antimicrob. Agents Chemother. (2002) [Pubmed]
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