Disease relevance of oprI

• The lipoprotein I gene (oprI) of Pseudomonas aeruginosa PAO1 was cloned and sequenced [1].
• After induction, E. coli cells harbouring the recombinant oprI gene became more sensitive to Cd and Co [2].
• Identification of African swine fever virus (ASFV) proteins recognised by cytotoxic T lymphocytes (CTL) from swine surviving ASFV/NH/P68 infection was assessed using expression vectors based on the Pseudomonas aeruginosa outer membrane lipoprotein I gene (oprI) [3].

High impact information on oprI

• Of 50 P. aeruginosa isolates that could be identified by basic biochemical testing, 100% were positive by real-time PCR with gyrB or oprI primers, 96% were positive with exotoxin A primers, and 92% were positive with algD primers [4].
• Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL [5].
• One OprF-based vaccine, called F/I, contains carboxy oprF sequences fused to oprI in an expression vector [6].
• The same cells, after IPTG induction, bound four to eight times more Cd and Cr than control cells expressing oprI alone [2].

Regulatory relationships of oprI

• Challenge with P. aeruginosa in a chronic pulmonary infection model demonstrated that boosting with the chimeric virus (but not with peptide-KLH) or adding oprI to the DNA vaccine significantly enhanced protection as compared to that afforded by the oprF vaccine given alone [7].

Analytical, diagnostic and therapeutic context of oprI

• Specific oligonucleotides were designed to amplify the oprI gene by the polymerase chain reaction (PCR) [1].
• Bacterial cells isolated by selective medium were identified by three methods: the presence of oprI and oprL, two outer membrane lipoprotein genes specific to P. aeruginosa; the API20 NE kit; and 16S rDNA sequence analysis [8].

References