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oprI  -  outer membrane lipoprotein OprI

Pseudomonas aeruginosa PAO1

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Disease relevance of oprI

  • The lipoprotein I gene (oprI) of Pseudomonas aeruginosa PAO1 was cloned and sequenced [1].
  • After induction, E. coli cells harbouring the recombinant oprI gene became more sensitive to Cd and Co [2].
  • Identification of African swine fever virus (ASFV) proteins recognised by cytotoxic T lymphocytes (CTL) from swine surviving ASFV/NH/P68 infection was assessed using expression vectors based on the Pseudomonas aeruginosa outer membrane lipoprotein I gene (oprI) [3].

High impact information on oprI

  • Of 50 P. aeruginosa isolates that could be identified by basic biochemical testing, 100% were positive by real-time PCR with gyrB or oprI primers, 96% were positive with exotoxin A primers, and 92% were positive with algD primers [4].
  • Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL [5].
  • One OprF-based vaccine, called F/I, contains carboxy oprF sequences fused to oprI in an expression vector [6].
  • The same cells, after IPTG induction, bound four to eight times more Cd and Cr than control cells expressing oprI alone [2].

Regulatory relationships of oprI

  • Challenge with P. aeruginosa in a chronic pulmonary infection model demonstrated that boosting with the chimeric virus (but not with peptide-KLH) or adding oprI to the DNA vaccine significantly enhanced protection as compared to that afforded by the oprF vaccine given alone [7].

Analytical, diagnostic and therapeutic context of oprI

  • Specific oligonucleotides were designed to amplify the oprI gene by the polymerase chain reaction (PCR) [1].
  • Bacterial cells isolated by selective medium were identified by three methods: the presence of oprI and oprL, two outer membrane lipoprotein genes specific to P. aeruginosa; the API20 NE kit; and 16S rDNA sequence analysis [8].


  1. Specificity of the Pseudomonas aeruginosa PAO1 lipoprotein I gene as a DNA probe and PCR target region within the Pseudomonadaceae. Saint-Onge, A., Romeyer, F., Lebel, P., Masson, L., Brousseau, R. J. Gen. Microbiol. (1992) [Pubmed]
  2. In-frame fusion of a His-Cys motif into the Pseudomonas aeruginosa outer membrane OprI lipoprotein results in increased metal binding capacity by Escherichia coli. Bouia, A., Kholti, A., Saghi, M., Cornelis, P. Res. Microbiol. (2001) [Pubmed]
  3. Identification of a 25-aminoacid sequence from the major African swine fever virus structural protein VP72 recognised by porcine cytotoxic T lymphocytes using a lipoprotein based expression system. Leitão, A., Malur, A., Cornelis, P., Martins, C.L. J. Virol. Methods (1998) [Pubmed]
  4. Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other nonfermenting gram-negative bacilli from patients with cystic fibrosis. Qin, X., Emerson, J., Stapp, J., Stapp, L., Abe, P., Burns, J.L. J. Clin. Microbiol. (2003) [Pubmed]
  5. Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL. De Vos, D., Lim, A., Pirnay, J.P., Struelens, M., Vandenvelde, C., Duinslaeger, L., Vanderkelen, A., Cornelis, P. J. Clin. Microbiol. (1997) [Pubmed]
  6. DNA vaccines against chronic lung infections by Pseudomonas aeruginosa. Staczek, J., Gilleland, L.B., van der Heyde, H.C., Gilleland, H.E. FEMS Immunol. Med. Microbiol. (2003) [Pubmed]
  7. Enhancement of the protective efficacy of an oprF DNA vaccine against Pseudomonas aeruginosa. Price, B.M., Barten Legutki, J., Galloway, D.R., von Specht, B.U., Gilleland, L.B., Gilleland, H.E., Staczek, J. FEMS Immunol. Med. Microbiol. (2002) [Pubmed]
  8. Pseudomonas aeruginosa isolated from marine environments in Tokyo Bay. Kimata, N., Nishino, T., Suzuki, S., Kogure, K. Microb. Ecol. (2004) [Pubmed]
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