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Gene Review

oprL  -  peptidoglycan associated lipoprotein OprL...

Pseudomonas aeruginosa PAO1

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Disease relevance of oprL

  • Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL [1].
  • Another ORF was found upstream of oprL, with homology to the TolB protein of Escherichia coli and Haemophilus influenzae [2].

High impact information on oprL

  • A set of data consisting of the nucleotide sequence of the oprL gene, a DNA-based fingerprint (amplified fragment length polymorphism, AFLP), serotype, pyoverdine type and antibiogram (MICs of 21 clinically relevant antibiotics) was assembled [3].
  • In addition to the AFLP pattern and serotype, analysis of the nucleotide sequences of three (lipo)protein genes (oprI, oprL, and oprD) and the pyoverdine type revealed that all predominant strains except the SSD(r) strain belonged to recently identified clonal complexes [4].
  • The first operon, orf1-tolQRA, is iron regulated throughout growth, but iron-regulated expression of tolB and oprL fusions occurs only in late log phase [5].
  • PCR analysis using specific primers for pAK1900 sequences and a chromosomal target, oprL, indicated that only plasmid DNA was contained within the lumen of p-MVs after exogenous DNA was digested by DNase [6].
  • Restriction mapping uncovered an 8.9 kb deletion of PAO sequence between phnAB and oprL in clone K, and two 106 kb insertions either adjacent to this deletion or several hundred kilobases away, close to the pilA locus [7].

Anatomical context of oprL

  • CONCLUSIONS: This study indicates that molecular detection of PA in sputum employing the oprL gene target, is a useful technique in the early detection of PA, gaining on average 4.5 months over conventional culture [8].

Analytical, diagnostic and therapeutic context of oprL

  • Bacterial cells isolated by selective medium were identified by three methods: the presence of oprI and oprL, two outer membrane lipoprotein genes specific to P. aeruginosa; the API20 NE kit; and 16S rDNA sequence analysis [9].


  1. Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL. De Vos, D., Lim, A., Pirnay, J.P., Struelens, M., Vandenvelde, C., Duinslaeger, L., Vanderkelen, A., Cornelis, P. J. Clin. Microbiol. (1997) [Pubmed]
  2. Molecular and immunological characterization of OprL, the 18 kDa outer-membrane peptidoglycan-associated lipoprotein (PAL) of Pseudomonas aeruginosa. Lim, A., De Vos, D., Brauns, M., Mossialos, D., Gaballa, A., Qing, D., Cornelis, P. Microbiology (Reading, Engl.) (1997) [Pubmed]
  3. Global Pseudomonas aeruginosa biodiversity as reflected in a Belgian river. Pirnay, J.P., Matthijs, S., Colak, H., Chablain, P., Bilocq, F., Van Eldere, J., De Vos, D., Zizi, M., Triest, L., Cornelis, P. Environ. Microbiol. (2005) [Pubmed]
  4. Molecular epidemiology of Pseudomonas aeruginosa colonization in a burn unit: persistence of a multidrug-resistant clone and a silver sulfadiazine-resistant clone. Pirnay, J.P., De Vos, D., Cochez, C., Bilocq, F., Pirson, J., Struelens, M., Duinslaeger, L., Cornelis, P., Zizi, M., Vanderkelen, A. J. Clin. Microbiol. (2003) [Pubmed]
  5. RegA, iron, and growth phase regulate expression of the Pseudomonas aeruginosa tol-oprL gene cluster. Duan, K., Lafontaine, E.R., Majumdar, S., Sokol, P.A. J. Bacteriol. (2000) [Pubmed]
  6. DNA-containing membrane vesicles of Pseudomonas aeruginosa PAO1 and their genetic transformation potential. Renelli, M., Matias, V., Lo, R.Y., Beveridge, T.J. Microbiology (Reading, Engl.) (2004) [Pubmed]
  7. Monitoring genome evolution ex vivo: reversible chromosomal integration of a 106 kb plasmid at two tRNA(Lys) gene loci in sequential Pseudomonas aeruginosa airway isolates. Kiewitz, C., Larbig, K., Klockgether, J., Weinel, C., Tümmler, B. Microbiology (Reading, Engl.) (2000) [Pubmed]
  8. Early detection of Pseudomonas aeruginosa--comparison of conventional versus molecular (PCR) detection directly from adult patients with cystic fibrosis (CF). Xu, J., Moore, J.E., Murphy, P.G., Millar, B.C., Elborn, J.S. Ann. Clin. Microbiol. Antimicrob. (2004) [Pubmed]
  9. Pseudomonas aeruginosa isolated from marine environments in Tokyo Bay. Kimata, N., Nishino, T., Suzuki, S., Kogure, K. Microb. Ecol. (2004) [Pubmed]
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