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Gene Review:

ECs4888 - acetylglutamate kinase

Escherichia coli O157:H7 str. Sakai

van Gorcom, R.F. et al., Penttilä, M. et al.

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Disease relevance of ECs4888

To analyse gene expression signals in Aspergillus we have constructed a set of integration vectors each of which contains in front of the Escherichia coli 'lacZ gene a unique BamHI site in one of the three possible translational reading frames and the A. nidulans argB gene as a selection marker [1].

High impact information on ECs4888

Transformation was obtained with plasmid carrying the dominant selectable marker amdS or the argB gene of Aspergillus nidulans, which was found to complement the respective argB mutation of T. reesei [2].
The insertion of the fusion genes at the argB locus assures the constancy of influences of the chromosomal environment on gene expression [1].
After transformation of an A. nidulans argB strain, the vectors integrate with a high percentage at the argB locus of the genome, as a single copy [1].

References

A system for the analysis of expression signals in Aspergillus. van Gorcom, R.F., Punt, P.J., Pouwels, P.H., van den Hondel, C.A. Gene (1986) [Pubmed]
A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. Penttilä, M., Nevalainen, H., Rättö, M., Salminen, E., Knowles, J. Gene (1987) [Pubmed]

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