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Gene Review:

rsmH - 16S rRNA m(4)C1402 methyltransferase, SAM...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0083, JW0080, mraW, yabC

Carrión, M. et al., Sommer, P. et al., Morel, F. et al., Kersulyte, D. et al., Sekine, Y. et al.

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Disease relevance of mraW

mraW, an essential gene at the dcw cluster of Escherichia coli codes for a cytoplasmic protein with methyltransferase activity [1].
One ORF (orfB) exhibited protein-level homology to one of two putative transposase genes found in several other chimeric elements including IS605 (also of H. pylori) and IS1535 (of Mycobacterium tuberculosis) [2].

High impact information on mraW

Downstream of lipA and in the opposite orientation, an open reading frame, orfB (encoding a 199-amino-acid protein) is found, which shows no conspicuous sequence similarity to known proteins, other than an NAD and flavin adenine dinucleotide binding-site sequence [3].
OrfAB is a transframe protein produced by -1 translational frameshifting between orfA and orfB, and it is known to be IS3 transposase [4].
We have found that mraW and mraZ are active genes, coding for two proteins with relative molecular masses of 34 800 and 17 300, respectively [1].

Biological context of mraW

Two open reading frames (orfB and orfC) were identified in the upstream region of the hsp18 gene, encoding the small heat-shock protein Lo18 [5].

Analytical, diagnostic and therapeutic context of mraW

METHODS AND RESULTS: Sequence analysis showed that orfB encodes a putative transcriptional regulator of the LysR family [5].

References

mraW, an essential gene at the dcw cluster of Escherichia coli codes for a cytoplasmic protein with methyltransferase activity. Carrión, M., Gómez, M.J., Merchante-Schubert, R., Dongarrá, S., Ayala, J.A. Biochimie (1999) [Pubmed]
Functional organization and insertion specificity of IS607, a chimeric element of Helicobacter pylori. Kersulyte, D., Mukhopadhyay, A.K., Shirai, M., Nakazawa, T., Berg, D.E. J. Bacteriol. (2000) [Pubmed]
Genetic and biochemical characterization of a new extracellular lipase from Streptomyces cinnamomeus. Sommer, P., Bormann, C., Götz, F. Appl. Environ. Microbiol. (1997) [Pubmed]
Inhibition of transpositional recombination by OrfA and OrfB proteins encoded by insertion sequence IS3. Sekine, Y., Izumi, K., Mizuno, T., Ohtsubo, E. Genes Cells (1997) [Pubmed]
Improved acid tolerance of a recombinant strain of Escherichia coli expressing genes from the acidophilic bacterium Oenococcus oeni. Morel, F., Delmas, F., Jobin, M.P., Diviès, C., Guzzo, J. Lett. Appl. Microbiol. (2001) [Pubmed]

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