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Gene Review:

rusA - DLP12 prophage; endonuclease RUS

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0541, JW0538, rus, ybcP

Mahdi, A.A. et al., Appia-Ayme, C. et al., Sharples, G.J. et al.

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Disease relevance of rusA

We show that rusA-like sequences are indeed often associated with prophage sequences in the genomes of several bacterial species [1].
Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82 [2].

High impact information on rusA

However, rusA is also present in the chromosome of the hyperthermophilic bacterium Aquifex aeolicus [1].
Recombinant plasmids carrying rusA were identified by their ability to make ruv mutants resistant to UV light [2].
The E. coli operon forms part of the defective lambdoid prophage, DLP12, and is probably derived from a phage related to 82 and PA-2. rusA appears to be very poorly expressed in E. coli, but can be activated by insertion of IS2 or IS10 upstream of the coding sequence to promote transcription [2].
These genes are in a locus containing four other genes: cyc2, which encodes a high-molecular-weight cytochrome c; cyc1, which encodes a c(4)-type cytochrome (c(552)); open reading frame 1, which encodes a putative periplasmic protein of unknown function; and rus, which encodes rusticyanin [3].

References

RusA proteins from the extreme thermophile Aquifex aeolicus and lactococcal phage r1t resolve Holliday junctions. Sharples, G.J., Bolt, E.L., Lloyd, R.G. Mol. Microbiol. (2002) [Pubmed]
Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82. Mahdi, A.A., Sharples, G.J., Mandal, T.N., Lloyd, R.G. J. Mol. Biol. (1996) [Pubmed]
Characterization of an operon encoding two c-type cytochromes, an aa(3)-type cytochrome oxidase, and rusticyanin in Thiobacillus ferrooxidans ATCC 33020. Appia-Ayme, C., Guiliani, N., Ratouchniak, J., Bonnefoy, V. Appl. Environ. Microbiol. (1999) [Pubmed]

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