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Gene Review:

rsuA - 16S rRNA U516 pseudouridine synthase

Escherichia coli str. K-12 substr. MG1655

Conrad, J. et al., Niu, L. et al.

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Disease relevance of rsuA

Therefore, rsuA is the only gene in E. coli with the ability to produce a protein capable of forming pseudouridine516 [1].
A Bacillus subtilis ORF, ypul, 41% homologous to rsuA, the gene for the synthase which forms pseudouridine 516 in Escherichia coli 16S rRNA, was cloned and the protein expressed and affinity-purified by the His tag procedure [2].

High impact information on rsuA

There was no effect on the growth rate of rsuA- MG1655 either in rich or minimal medium at either 24, 37, or 42 degrees C. Plasmid rescue of the BL21/DE3 rsuA- strain using pET15b containing an rsuA gene with aspartate102 replaced by asparagine or threonine demonstrated that neither mutant was active in vivo [1].

References

16S ribosomal RNA pseudouridine synthase RsuA of Escherichia coli: deletion, mutation of the conserved Asp102 residue, and sequence comparison among all other pseudouridine synthases. Conrad, J., Niu, L., Rudd, K., Lane, B.G., Ofengand, J. RNA (1999) [Pubmed]
Cloning and characterization of the 23S RNA pseudouridine 2633 synthase from Bacillus subtilis. Niu, L., Lane, B.G., Ofengand, J. Biochemistry (1999) [Pubmed]

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