Disease relevance of flhD

• Another stock of MG1655 was obtained from the E. coli Genetic Stock Center. This stock contained isolates which were poorly motile and had no IS1 element upstream of the flhD promoter [1].

High impact information on flhD

• Expression of the flbB/flaI operon, thought to encode sigma F, is decreased in apaH- mutants, and there appears to be a general decrease in expression of genes regulated by cAMP-binding protein and cAMP as well [2].
• QseBC activates transcription of flhDC, which is the master regulator for the flagella and motility genes and, in the absence of flhD, QseBC failed to activate the transcription of fliA [3].
• The flhD operon is the master operon of the flagellar regulon and a global regulator of metabolism [1].
• Increased motility of Escherichia coli by insertion sequence element integration into the regulatory region of the flhD operon [1].
• The genome sequence of the Escherichia coli K-12 strain MG1655 contained an IS1 insertion sequence element in the regulatory region of the flhD promoter [1].

Biological context of flhD

• We suggest that IS element insertion may activate transcription of the flhD operon by reducing transcriptional repression [1].
• Four other strains (W1485, FB8, MM294, and RB791) did not contain IS elements in the flhD regulatory region and were poorly motile [1].

References