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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

rnb  -  ribonuclease II

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1281, JW1279
 
 
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Disease relevance of rnb

  • Genetic analysis of these mutants by Hfr conjugation and P1 transduction indicates that the structural gene (rnb) for ribonuclease II is located near the pyrF gene (28 min on the E. coli genetic map of Bachmann, Low and Taylor (1976)), and the most probable gene order is tyrT-trp-pyrF-rnb [1].
 

High impact information on rnb

  • Translation initiation was confirmed by rnb-lacZ fusions [2].
  • Sequencing of a 2.2 kb MseI-RsaI fragment containing the rnb gene revealed an open reading frame of 1794 nucleotides that encodes a protein of 598 amino acid residues, whose calculated molecular mass is 67,583 Da [2].
  • We report the precise physical mapping of rnb to 29 min on the chromosomal map in the vicinity of pyrF, and clarify the genetic and physical maps of this E. coli chromosomal region [3].
  • Ribonuclease II (encoded by rnb) is one of the two main exonucleases involved in mRNA degradation in Escherichia coli [3].
  • We have constructed strain CMA201, in which the rnb promoter region and the gene were deleted from the chromosome and replaced by a tetr cassette [4].

References

  1. Genetic analysis of mutations affecting ribonuclease II in Escherichia coli. Ono, M., Kuwano, M. Mol. Gen. Genet. (1977) [Pubmed]
  2. DNA sequencing and expression of the gene rnb encoding Escherichia coli ribonuclease II. Zilhão, R., Camelo, L., Arraiano, C.M. Mol. Microbiol. (1993) [Pubmed]
  3. Precise physical mapping of the Escherichia coli rnb gene, encoding ribonuclease II. Zilhão, R., Caillet, J., Régnier, P., Arraiano, C.M. Mol. Gen. Genet. (1995) [Pubmed]
  4. Construction and characterization of an absolute deletion mutant of Escherichia coli ribonuclease II. Piedade, J., Zilhão, R., Arraiano, C.M. FEMS Microbiol. Lett. (1995) [Pubmed]
 
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