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Gene Review

pyrF  -  orotidine-5'-phosphate decarboxylase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1276, JW1273
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Disease relevance of pyrF


High impact information on pyrF


Chemical compound and disease context of pyrF

  • Stx- mutants of an Stx+ E. coli transductant were generated by random in vivo insertion mutagenesis with a Tn10 derivative transposon, Tn-mini-kan, followed by P1 cotransduction of the kanamycin resistance and PyrF+ markers into a pyrF Stx+ E. coli K-12 recipient [2].

Biological context of pyrF

  • The primary transcriptional initiation site is 51 base pairs upstream of the pyrF structural gene [1].
  • Complementation of E. coli pyrD, pyrF and pyrE mutants was obtained with a L. plantarum genomic DNA library [6].
  • By integrating an F'lac episome into the lac part of the fusions and determining the direction of chromosomal transfer from the resultant Hfr strains, the direction of pyrC transcription was found to be counter-clockwise, while pyrD and pyrF were found to be transcribed in a clockwise direction [7].
  • Genetic analysis of these mutants by Hfr conjugation and P1 transduction indicates that the structural gene (rnb) for ribonuclease II is located near the pyrF gene (28 min on the E. coli genetic map of Bachmann, Low and Taylor (1976)), and the most probable gene order is tyrT-trp-pyrF-rnb [8].
  • A gene that specifies high-level Shiga toxin production is thus located near pyrF on the chromosome of S. dysenteriae 1 [2].

Associations of pyrF with chemical compounds


Other interactions of pyrF


Analytical, diagnostic and therapeutic context of pyrF


  1. Nucleotide sequence and characterization of the pyrF operon of Escherichia coli K12. Turnbough, C.L., Kerr, K.H., Funderburg, W.R., Donahue, J.P., Powell, F.E. J. Biol. Chem. (1987) [Pubmed]
  2. Localization of stx, a determinant essential for high-level production of shiga toxin by Shigella dysenteriae serotype 1, near pyrF and generation of stx transposon mutants. Sekizaki, T., Harayama, S., Brazil, G.M., Timmis, K.N. Infect. Immun. (1987) [Pubmed]
  3. Orotidine-5'-monophosphate decarboxylase from Pseudomonas aeruginosa PAO1: cloning, overexpression, and enzyme characterization. Strych, U., Wohlfarth, S., Winkler, U.K. Curr. Microbiol. (1994) [Pubmed]
  4. Selection of catalytic antibodies for a biosynthetic reaction from a combinatorial cDNA library by complementation of an auxotrophic Escherichia coli: antibodies for orotate decarboxylation. Smiley, J.A., Benkovic, S.J. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  5. Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli. Bach, M.L., Lacroute, F., Botstein, D. Proc. Natl. Acad. Sci. U.S.A. (1979) [Pubmed]
  6. Structure and organisation of the pyrimidine biosynthesis pathway genes in Lactobacillus plantarum: a PCR strategy for sequencing without cloning. Elagöz, A., Abdi, A., Hubert, J.C., Kammerer, B. Gene (1996) [Pubmed]
  7. Studies on the structure and expression of Escherichia coli pyrC, pyrD, and pyrF using the cloned genes. Jensen, K.F., Larsen, J.N., Schack, L., Sivertsen, A. Eur. J. Biochem. (1984) [Pubmed]
  8. Genetic analysis of mutations affecting ribonuclease II in Escherichia coli. Ono, M., Kuwano, M. Mol. Gen. Genet. (1977) [Pubmed]
  9. Sequence analysis and identification of the pyrKDbF operon from Lactococcus lactis including a novel gene, pyrK, involved in pyrimidine biosynthesis. Andersen, P.S., Martinussen, J., Hammer, K. J. Bacteriol. (1996) [Pubmed]
  10. Glutamate dehydrogenase: genetic mapping and isolation of regulatory mutants of Klebsiella aerogenes. Bender, R.A., Macaluso, A., Magasanik, B. J. Bacteriol. (1976) [Pubmed]
  11. The isolation of specific genes from the basidiomycete Schizophyllum commune. Froeliger, E.H., Muñoz-Rivas, A.M., Specht, C.A., Ullrich, R.C., Novotny, C.P. Curr. Genet. (1987) [Pubmed]
  12. Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation. Wright, D.A., Park, S.K., Wu, D., Phillips, G.J., Rodermel, S.R., Voytas, D.F. Nucleic Acids Res. (1997) [Pubmed]
  13. Cloning and characterization of ftsZ and pyrF from the archaeon Thermoplasma acidophilum. Yaoi, T., Laksanalamai, P., Jiemjit, A., Kagawa, H.K., Alton, T., Trent, J.D. Biochem. Biophys. Res. Commun. (2000) [Pubmed]
  14. Purification and characterization of orotidine-5'-phosphate decarboxylase from Escherichia coli K-12. Donovan, W.P., Kushner, S.R. J. Bacteriol. (1983) [Pubmed]
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