Disease relevance of dsbB

• Identification and characterization of the Escherichia coli gene dsbB, whose product is involved in the formation of disulfide bonds in vivo [1].
• Cloning and sequencing of nhaB gene encoding an Na+/H+ antiporter from Vibrio alginolyticus [2].

High impact information on dsbB

• The dsbG gene was cloned from a multicopy plasmid library lacking the dsbB redox protein-encoding gene [3].
• This DsbG defect could be rescued by addition to the growth medium of either oxidized dithiothreitol or cystine, or by overexpression of the dsbA or dsbB genes [3].
• Sequence analysis revealed that these revertants carried mutations in dsbB and that their Ps(+) phenotypes could be suppressed by the wild-type allele of dsbB [4].
• A gene has been cloned from the marine bacterium Vibrio alginolyticus that functionally complements a mutant strain of Escherichia coli, TO114, defective in three Na+/H+ antiport genes (nhaA, nhaB, chaA) [2].
• This gene has 62% identity to nhaB gene at the DNA level from Escherichia coli and the deduced amino acid sequence is 67% identical with E. coli NhaB [2].

Other interactions of dsbB

• To investigate the DsbA-DsbB catalytic system, we performed an in vivo selection for chromosomal dsbA and dsbB mutants [5].

References