Disease relevance of ansB

• The ansB gene maps to position 3114 kb on the physical map of E. coli [Kohara et al., Cell 50 (1987) 495-508], corresponding to approx. 63.8 min on the genetic map [1].
• Similarly, the homologous ansB promoter in Salmonella is co-activated by two dimers of CRP which function synergistically [2].

High impact information on ansB

• The ansB promoter in Escherichia coli is co-activated in a co-dependent manner by one dimer each of CRP and FNR protein whose binding sites are at around -91 and -41 bp, respectively, from the transcription start site [2].
• The nucleotide sequence of ansB, including 5'- and 3'-untranslated regions, was determined [3].
• The transcriptional start site in ansB was determined, allowing the identification of the promoter region [3].
• The most efficient of these systems consists of plasmid pTWE1, a derivative of pT7-7, and an ansB- strain of E. coli, CU1783 [4].

Analytical, diagnostic and therapeutic context of ansB

• The Escherichia coli gene ansB, encoding the chemotherapeutic enzyme L-asparaginase II, has been cloned, using a strategy based on the polymerase chain reaction, and sequenced [1].

References