Disease relevance of lysU

• Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species [1].

High impact information on lysU

• In contrast to the recent proposal of Gampel and Tzagoloff (1989, Proc. Natl. Acad. Sci. USA 86, 6023-6027), the lysU sequence is distinct from the open reading frame located adjacent to frdA, although large homologies are shared by these two genes [1].
• These mutations nullified the hns gene encoding a histone-like protein, H-NS, and affected thermoregulation of lysU [2].
• A novel class of lysU-constitutive mutations were isolated by random insertion mutagenesis [2].
• Accumulation of high levels of LysU during growth of strains carrying the wild-type allele of lysU on multicopy plasmids had no observable effect on growth or on the synthesis of LysS [3].
• Pyridoxal 5'-phosphate (PLP) and pyridoxal 5'-diphosphate (PLDP) were used to identify lysyl residues at the phosphate-binding locus in the lysS-encoded and the lysU-encoded lysyl-tRNA synthetases (LysRSs and LysRSu, respectively) from Escherichia coli [4].

Chemical compound and disease context of lysU

• The first threedimensional structure of a lysyl-tRNA synthetase worked out very recently, using the enzyme from the Escherichia coli lysU gene which binds one molecule of lysine, is similar to those of other class II synthetases [5].

References