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Gene Review:

ORF1 - 145-kDa protein

Sorghum chlorotic spot virus

Koenig, R. et al., Herzog, E. et al., Locali-Fabris, E.C. et al., Manohar, S.K. et al.

Koenig, Loss, Locali-Fabris, Freitas-Astúa, Souza, Takita, Astúa-Monge, Antonioli-Luizon, Rodrigues, Targon, Machado,

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Disease relevance of ORF1

Beet soil-borne virus RNA 1: genetic analysis enabled by a starting sequence generated with primers to highly conserved helicase-encoding domains [1].

High impact information on ORF1

The 286 kDa protein is a polyprotein putatively involved in virus replication, which contains four conserved domains: methyltransferase, protease, helicase and polymerase [2].
The cistron for the coat protein (ORF 1) is located nearest the 5' end of the RNA and is separated from the 5' extremity by a noncoding region of 389 nucleotides [3].

Biological context of ORF1

The 5'-terminal proximal ORF has the potential to encode a polypeptide of M(r) 130942 (P131) containing methyltransferase and RNA helicase homologous domains and displaying homology with large nonstructural proteins of alpha-like viruses, which are known or thought to be involved in virus replication [4].

Analytical, diagnostic and therapeutic context of ORF1

The complete sequence of the 5834 nucleotides of RNA 1 of beet soil-borne furovirus (BSBV, Ahlum isolate) was determined using a PCR product obtained with primers to highly conserved coding regions for helicase-like proteins in RNA 1 of furo-, hordei- and tobraviruses as a starting sequence [1].

References

Beet soil-borne virus RNA 1: genetic analysis enabled by a starting sequence generated with primers to highly conserved helicase-encoding domains. Koenig, R., Loss, S. J. Gen. Virol. (1997) [Pubmed]
Complete nucleotide sequence, genomic organization and phylogenetic analysis of Citrus leprosis virus cytoplasmic type. Locali-Fabris, E.C., Freitas-Astúa, J., Souza, A.A., Takita, M.A., Astúa-Monge, G., Antonioli-Luizon, R., Rodrigues, V., Targon, M.L., Machado, M.A. J. Gen. Virol. (2006) [Pubmed]
Nucleotide sequence and genetic organization of peanut clump virus RNA 2 and partial characterization of deleted forms. Manohar, S.K., Guilley, H., Dollet, M., Richards, K., Jonard, G. Virology (1993) [Pubmed]
Complete nucleotide sequence of peanut clump virus RNA 1 and relationships with other fungus-transmitted rod-shaped viruses. Herzog, E., Guilley, H., Manohar, S.K., Dollet, M., Richards, K., Fritsch, C., Jonard, G. J. Gen. Virol. (1994) [Pubmed]

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