Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

ligA - NAD-dependent DNA ligase LigA

Escherichia coli O157:H7 str. EDL933

Seo, M.S. et al., Cheng, C. et al., Koizumi, N. et al.

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Disease relevance of lig

Characterization of an ATP-dependent DNA ligase encoded by Haemophilus influenzae [1].
The Sma DNA ligase gene was expressed under the control of the T7lac promoter on the pET-22b(+) in Escherichia coli BL21-CodonPlus(DE3)-RIL [2].

High impact information on lig

The Haemophilus enzyme is the first example of an ATP-dependent DNA ligase encoded by a eubacterial genome [1].
Upon alignment of the entire amino acid sequence, Sma DNA ligase showed a high degree of sequence homology with the hyperthemophilic archaeal DNA ligases, 67% identity with Aeropyrum pernix K1, and 40% identity with both Pyrococcus abyssi and Thermococcus kodakarensis [2].

Analytical, diagnostic and therapeutic context of lig

Southern blot analysis indicated the conservation of the lig genes among pathogenic leptospires [3].

References

Characterization of an ATP-dependent DNA ligase encoded by Haemophilus influenzae. Cheng, C., Shuman, S. Nucleic Acids Res. (1997) [Pubmed]
Cloning and expression of a DNA ligase from the hyperthermophilic archaeon Staphylothermus marinus and properties of the enzyme. Seo, M.S., Kim, Y.J., Choi, J.J., Lee, M.S., Kim, J.H., Lee, J.H., Kwon, S.T. J. Biotechnol. (2007) [Pubmed]
Leptospiral immunoglobulin-like proteins elicit protective immunity. Koizumi, N., Watanabe, H. Vaccine (2004) [Pubmed]

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