High impact information on Chromosomes, Artificial, Human

• A human artificial chromosome (HAC) vector was constructed from a 1-Mb yeast artificial chromosome (YAC) that was selected based on its size from among several YACs identified by screening a randomly chosen subset of the Centre d'Etude du Polymorphisme Humain (CEPH) (Paris) YAC library with a degenerate alpha satellite probe [1].
• Haploid strains with the tlc1-human allele will provide an important tool for studying the function of telomerase and its regulation by telomere-binding proteins, and these strains will serve as good hosts for human artificial chromosome assembly and propagation [2].
• Functional expression of yeast artificial chromosome-human multidrug resistance genes in mouse cells [3].
• Chromatin immunoprecipitation analyses of the re-formed minichromosome and the alphoid YAC-based stable human artificial chromosome both indicated that CENP-A and CENP-B assembled only on the inserted alphoid array but not on the YAC arms [4].
• Generation of human artificial chromosomes expressing naturally controlled guanosine triphosphate cyclohydrolase I gene [5].

Biological context of Chromosomes, Artificial, Human

• The ability of the cloned human centromeric regions to support human artificial chromosome (HAC) formation was assessed by transfection into human HT1080 cells [6].

Associations of Chromosomes, Artificial, Human with chemical compounds

• We used the method to construct a human artificial chromosome vector of 404 kb encompassing long tracts of alpha satellite DNA, telomeric sequences, and the human hypoxanthine phosphoribosyltransferase gene [7].

Gene context of Chromosomes, Artificial, Human

• BACKGROUND: Human artificial chromosomes (HACs) are generated from the precursor DNA constructs containing alpha-satellite DNA with CENP-B boxes, and the process could be used for the incorporation of large genes in the HACs [5].

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