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Gene Review

CENPB  -  centromere protein B, 80kDa

Homo sapiens

Synonyms: CENP-B, Centromere protein B, Major centromere autoantigen B
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Disease relevance of CENPB

  • The DNA binding domain has been delimited within the NH2-terminal 125-amino acid region containing four potential alpha-helices using truncated CENP-B made in Escherichia coli cells [1].
  • The cDNA was inserted into a modified baculovirus transfer vector which mediated high-level expression of recombinant human CENP-B with a histidine-hexapeptide as affinity ligand at its N-terminus in infected Spodoptera frugiperda (Sf9) insect cells [2].
  • An ELISA established with the eukaryotically expressed and purified recombinant human full-length CENP-B demonstrated its excellent specificity, sensitivity and reproducibility for the measurement of autoantibodies directed to the human CENP-B (ACA-B) representing a diagnostic marker for CREST syndrome, an autoimmune rheumatic disease [2].
  • Development of a CENP-A/CENP-B-specific immune response in a patient with systemic sclerosis [3].
  • The immediate-early (IE) protein of HSV-1, which activates HIV-1 regulatory functions, contains three homologies to the 70-kDa antigen (two hexamers and a pentamer) and two to CENP-B (a hexamer and pentamer) [4].

Psychiatry related information on CENPB

  • The idiotypes (Ids) of anticentromere antibodies (ACA) have been studied using a fusion protein obtained from cloned cDNA of the major centromere antigen, CENP-B, for isolation of the autoantibodies [5].

High impact information on CENPB


Biological context of CENPB


Anatomical context of CENPB

  • Expression of epitope-tagged deletion derivatives of CENP-B in HeLa cells revealed that a single domain less than 158 residues from the amino terminus of the protein is sufficient to localize CENP-B to centromeres [11].
  • Domains in the CENP-B polypeptide involved in the formation of complex A were determined in the present study with truncated derivatives expressed in Escherichia coli and in rabbit reticulocyte lysates [12].
  • CENP-B mRNA was detected in mouse cells and tissues and an immunoreactive nuclear protein identical in size to human CENP-B was detected in mouse 3T3 cells using human ACA [13].
  • Like CENP-B and C, but unlike CENP-A, this protein remained with the nuclear matrix after intensive extraction [14].
  • We have studied the ability of the two main human centromere proteins CENP-A (18 kD) and CENP-B (80 kD) to bind tubulin, in order to correlate with one of the putative functional roles in spindle microtubule attachment [15].

Associations of CENPB with chemical compounds

  • A human centromere protein, CENP-B, has a DNA binding domain containing four potential alpha helices at the NH2 terminus, which is separable from dimerizing activity [1].
  • The carboxy terminus of CENP-B contains two long domains comprised almost entirely of glutamic and aspartic acid residues [16].
  • The chromosome is negative for CBG banding and is devoid of detectable centromeric alpha satellite and its associated centromere protein CENP-B, suggesting activation of a neocentromere within the 1p32-36.1 region [17].
  • Sera were analyzed by immunoprecipitation of [35S] methionine-labeled Ro 52, La, and CENP B and C generated by coupled in vitro transcription/translation [18].

Physical interactions of CENPB

  • This result suggests that CENP-A nucleosomes form a complex with CENP-B and CENP-C through interaction with DNA [19].
  • Hamster CENP-B protein analysis revealed at the N-terminal region a 133 amino acid fragment of 100% homology to the DNA binding motif identified previously for the human autoantigen [20].

Regulatory relationships of CENPB


Other interactions of CENPB


Analytical, diagnostic and therapeutic context of CENPB


  1. A human centromere protein, CENP-B, has a DNA binding domain containing four potential alpha helices at the NH2 terminus, which is separable from dimerizing activity. Yoda, K., Kitagawa, K., Masumoto, H., Muro, Y., Okazaki, T. J. Cell Biol. (1992) [Pubmed]
  2. Eukaryotic expression of recombinant human centromere autoantigen and its use in a novel ELISA for diagnosis of CREST syndrome. Stahnke, G., Meier, E., Scanarini, M., Northemann, W. J. Autoimmun. (1994) [Pubmed]
  3. Development of a CENP-A/CENP-B-specific immune response in a patient with systemic sclerosis. Mahler, M., Mierau, R., Genth, E., Blüthner, M. Arthritis Rheum. (2002) [Pubmed]
  4. Multiple overlapping homologies between two rheumatoid antigens and immunosuppressive viruses. Douvas, A., Sobelman, S. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  5. Idiotypic analysis of human anticentromere autoantibodies. Hildebrandt, S., Weiner, E.S., Earnshaw, W.C., Zanetti, M., Rothfield, N.F. Autoimmunity (1991) [Pubmed]
  6. Fission yeast CENP-B homologs nucleate centromeric heterochromatin by promoting heterochromatin-specific histone tail modifications. Nakagawa, H., Lee, J.K., Hurwitz, J., Allshire, R.C., Nakayama, J., Grewal, S.I., Tanaka, K., Murakami, Y. Genes Dev. (2002) [Pubmed]
  7. CENP-B box is required for de novo centromere chromatin assembly on human alphoid DNA. Ohzeki, J., Nakano, M., Okada, T., Masumoto, H. J. Cell Biol. (2002) [Pubmed]
  8. A centromere DNA-binding protein from fission yeast affects chromosome segregation and has homology to human CENP-B. Halverson, D., Baum, M., Stryker, J., Carbon, J., Clarke, L. J. Cell Biol. (1997) [Pubmed]
  9. Centromeres, CENP-B and Tigger too. Kipling, D., Warburton, P.E. Trends Genet. (1997) [Pubmed]
  10. CENP-B: a major human centromere protein located beneath the kinetochore. Cooke, C.A., Bernat, R.L., Earnshaw, W.C. J. Cell Biol. (1990) [Pubmed]
  11. Identification of a subdomain of CENP-B that is necessary and sufficient for localization to the human centromere. Pluta, A.F., Saitoh, N., Goldberg, I., Earnshaw, W.C. J. Cell Biol. (1992) [Pubmed]
  12. Analysis of protein-DNA and protein-protein interactions of centromere protein B (CENP-B) and properties of the DNA-CENP-B complex in the cell cycle. Kitagawa, K., Masumoto, H., Ikeda, M., Okazaki, T. Mol. Cell. Biol. (1995) [Pubmed]
  13. CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins. Sullivan, K.F., Glass, C.A. Chromosoma (1991) [Pubmed]
  14. CENP-G: a new centromeric protein that is associated with the alpha-1 satellite DNA subfamily. He, D., Zeng, C., Woods, K., Zhong, L., Turner, D., Busch, R.K., Brinkley, B.R., Busch, H. Chromosoma (1998) [Pubmed]
  15. The centromere protein CENP-B behaves as a microtubule-associated protein. Armas-Portela, R., Kremer, L., Avila, J. Acta Histochem. Suppl. (1991) [Pubmed]
  16. Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen. Earnshaw, W.C., Sullivan, K.F., Machlin, P.S., Cooke, C.A., Kaiser, D.A., Pollard, T.D., Rothfield, N.F., Cleveland, D.W. J. Cell Biol. (1987) [Pubmed]
  17. Neocentromere formation in a stable ring 1p32-p36.1 chromosome. Slater, H.R., Nouri, S., Earle, E., Lo, A.W., Hale, L.G., Choo, K.H. J. Med. Genet. (1999) [Pubmed]
  18. Centromere protein C is a target of autoantibodies in Sjögren's syndrome and is uniformly associated with antibodies to Ro and La. Pillemer, S.R., Casciola-Rosen, L., Baum, B.J., Rosen, A., Gelber, A.C. J. Rheumatol. (2004) [Pubmed]
  19. CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells. Ando, S., Yang, H., Nozaki, N., Okazaki, T., Yoda, K. Mol. Cell. Biol. (2002) [Pubmed]
  20. Molecular cloning of an intronless gene for the hamster centromere antigen CENP-B. Bejarano, L.A., Valdivia, M.M. Biochim. Biophys. Acta (1996) [Pubmed]
  21. Nuclear autoantigen CENP-B transactivation of the epidermal growth factor receptor via chemokine receptor 3 in vascular smooth muscle cells. Robitaille, G., Christin, M.S., Clément, I., Senécal, J.L., Raymond, Y. Arthritis Rheum. (2009) [Pubmed]
  22. Assembly of additional heterochromatin distinct from centromere-kinetochore chromatin is required for de novo formation of human artificial chromosome. Nakashima, H., Nakano, M., Ohnishi, R., Hiraoka, Y., Kaneda, Y., Sugino, A., Masumoto, H. J. Cell. Sci. (2005) [Pubmed]
  23. Extreme reduction of chromosome-specific alpha-satellite array is unusually common in human chromosome 21. Lo, A.W., Liao, G.C., Rocchi, M., Choo, K.H. Genome Res. (1999) [Pubmed]
  24. 17-AAG, an Hsp90 inhibitor, causes kinetochore defects: a novel mechanism by which 17-AAG inhibits cell proliferation. Niikura, Y., Ohta, S., Vandenbeldt, K.J., Abdulle, R., McEwen, B.F., Kitagawa, K. Oncogene (2006) [Pubmed]
  25. Mapping of the human centromere protein B gene (CENPB) to chromosome 20p13 by fluorescence in situ hybridization. Seki, N., Saito, T., Kitagawa, K., Masumoto, H., Okazaki, T., Hori, T.A. Genomics (1994) [Pubmed]
  26. Three human chromosomal autoantigens are recognized by sera from patients with anti-centromere antibodies. Earnshaw, W., Bordwell, B., Marino, C., Rothfield, N. J. Clin. Invest. (1986) [Pubmed]
  27. Recognition of Granzyme B-generated autoantigen fragments in scleroderma patients with ischemic digital loss. Schachna, L., Wigley, F.M., Morris, S., Gelber, A.C., Rosen, A., Casciola-Rosen, L. Arthritis Rheum. (2002) [Pubmed]
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