Sensitivity of PCR assays for the determination of hepatitis A virus RNA in plasma pools. A collaborative study.
BACKGROUND AND OBJECTIVES: A collaborative study was organised to establish a hepatitis A virus (HAV) RNA standard for genomic amplification assays (GAT). MATERIALS AND METHODS: A panel of 10 samples consisting of a 10-fold serial dilution of wild-type HAV diluted in HAV-negative cryosupernatant and samples of the diluent were sent to 14 laboratories in duplicate for testing for HAV RNA by the polymerase chain reaction. RESULTS: Data returned by 12 laboratories indicated that the sensitivities of the assays performed by different laboratories were fairly close: there was a 100-fold difference in sensitivity between the majority of laboratories. Only one laboratory reported false-positive results. CONCLUSION: A 10-5 dilution of the virus in cryosupernatant could be an appropriate working reagent for GAT assays for HAV RNA in plasma pools.[1]References
- Sensitivity of PCR assays for the determination of hepatitis A virus RNA in plasma pools. A collaborative study. Saldanha, J. Vox Sang. (1999) [Pubmed]
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