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PHA production, from bacteria to plants.

The genes encoding the polyhydroxyalkanoate (PHA) biosynthetic pathway in Ralstonia eutropha (3-ketothiolase, phaA or bktB; acetoacetyl-CoA reductase, phaB; and PHA synthase, phaC) were engineered for plant plastid targeting and expressed using leaf (e35S) or seed-specific (7s or lesquerella hydroxylase) promoters in Arabidopsis and Brassica. PHA yields in homozygous transformants were 12-13% of the dry mass in homozygous Arabidopsis plants and approximately 7% of the seed weight in seeds from heterozygous canola plants. When a threonine deaminase was expressed in addition to bktB, phaB and phaC, a copolyester of 3-hydroxybutyrate and 3-hydroxyvalerate was produced in both Arabidopsis and Brassica.[1]

References

  1. PHA production, from bacteria to plants. Valentin, H.E., Broyles, D.L., Casagrande, L.A., Colburn, S.M., Creely, W.L., DeLaquil, P.A., Felton, H.M., Gonzalez, K.A., Houmiel, K.L., Lutke, K., Mahadeo, D.A., Mitsky, T.A., Padgette, S.R., Reiser, S.E., Slater, S., Stark, D.M., Stock, R.T., Stone, D.A., Taylor, N.B., Thorne, G.M., Tran, M., Gruys, K.J. Int. J. Biol. Macromol. (1999) [Pubmed]
 
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