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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei.

We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C ( PC- PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The deduced amino acid sequence exhibited 48% similarity to that of a nonhemolytic PLC from Pseudomonas aeruginosa. The expressed PC- PLC was heat stable, nonhemolytic for sheep erythrocytes, and active between pH 2 and 8. Western blot analysis with sera from melioidosis patients indicated that they produced immunoglobulin M antibodies against this PC- PLC protein.[1]

References

  1. Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei. Korbsrisate, S., Suwanasai, N., Leelaporn, A., Ezaki, T., Kawamura, Y., Sarasombath, S. J. Clin. Microbiol. (1999) [Pubmed]
 
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