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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Translation of Semliki forest virus 42S and 26S RNAs in a cell-free system derived from Escherichia coli.

The SFV 42S RNA and the intracellular 26S RNA have been translated in a prokaryotic cell-free system, the E. coli S30. About half of the [35S]methionine-labelled products directed by both RNAs had molecular weights larger than 20,000 on polyacrylamide gels. Both products contained tryptic peptides which comigrated with all the capsid and envelope protein-derived peptides. The most striking difference between the prokaryotic and eukaryotic systems lay in the translation of the 42 S RNA: The "42S RNA-specific nonstructural" peptides, which predominate in the eukaryotic systems, were apparently absent from the product translated by the prokaryotic system.[1]

References

  1. Translation of Semliki forest virus 42S and 26S RNAs in a cell-free system derived from Escherichia coli. Uomala, P., Glanville, N., Morser, J., Kääriäinen, L. Med. Biol. (1975) [Pubmed]
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