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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

p-Hydroxyphenylacetic Acid Metabolism in Pseudomonas putida F6.

Pseudomonas putida F6 was found to metabolize p-hydroxyphenylacetic acid through 3,4-dihydroxyphenylacetic acid, 3,4-dihydroxymandelic acid, and 3,4-dihydroxybenzaldehyde. Cell extracts of P. putida F6 catalyze the NAD(P)H-independent hydroxylation of p-hydroxyphenylacetic acid to 3,4-dihydroxyphenylacetic acid which is further oxidized to 3,4-dihydroxymandelic acid. Oxidation and decarboxylation of the latter yields 3,4-dihydroxybenzaldehyde. A red-brown color accompanies all of the above enzyme activities and is probably due to the polymerization of quinone-like compounds. 3,4-Dihydroxybenzaldehyde is further metabolized through extradiol ring cleavage.[1]


  1. p-Hydroxyphenylacetic Acid Metabolism in Pseudomonas putida F6. O'Connor, K.E., Witholt, B., Duetz, W. J. Bacteriol. (2001) [Pubmed]
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