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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants.

The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.[1]

References

  1. New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants. Wagner, R., Straub, M.L., Souciet, J.L., Potier, S., de Montigny, J. J. Bacteriol. (2001) [Pubmed]
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