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Gene Review:

FCY2 - Fcy2p

Saccharomyces cerevisiae S288c

Synonyms: Cytosine/purine transport protein FCY2, Fluorocytosine resistance protein 2, PCP FCY2, Purine-cytosine permease FCY2, YER056C

Paluszynski, J.P. et al., Grandier-Vazeille, X. et al., Weber, E. et al., Wagner, R. et al., Pinson, B. et al., et al.

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Disease relevance of FCY2

By applying a highly sensitive assay, a low-level but dose-dependent toxicity of 5-FC in fcy2 mutants was detected, whereas cells deficient in the cytosine deaminase (encoded by FCY1), which is essential for intracellular conversion of 5-FC to 5-fluorouracil, display strong dose-independent resistance [1].

High impact information on FCY2

The FCY2 gene was mapped on the right arm of chromosome V close to the HIS1 gene [2].
Expression of the FCY2 gene product requires a functional secretory pathway and is reduced in mnn9, a mutant strain deficient in outer chain glycosylation [2].
BRA7 is allelic to FCY2, the gene encoding the purine cytosine permease and BRA9 is ADE12, the gene encoding adenylosuccinate synthetase [3].
The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine [4].
A genome-wide search for cytosine permease homologues identified two uncharacterized candidate genes, designated FCY21 and FCY22, both of which exhibit highest similarity to FCY2 [1].

Biological context of FCY2

The corresponding plasmids were introduced into a S. cerevisiae strain carrying a chromosomal deletion at the FCY2 locus [5].
A synthetic peptide, selected in the predicted N-terminal amino-acid sequence of the purine cytosine permease (gene FCY2), linked to albumins proved a remarkably good immunogen in rabbits [6].

Anatomical context of FCY2

The purine/cytosine permease, encoded by the FCY2 gene, is a carrier located in the plasma membrane of the yeast Saccharomyces cerevisiae [7].

Associations of FCY2 with chemical compounds

5-FC uptake in S. cerevisiae was considered to be mediated by the FCY2-encoded cytosine/adenine permease [1].

Analytical, diagnostic and therapeutic context of FCY2

In ELISA, sera reacted with the synthetic peptide and with specific proteins of plasma-membrane-enriched fractions of mutant Saccharomyces cerevisiae pAB strains (amplified FCY2 gene) with high titers and high avidity [6].

References

Various cytosine/adenine permease homologues are involved in the toxicity of 5-fluorocytosine in Saccharomyces cerevisiae. Paluszynski, J.P., Klassen, R., Rohe, M., Meinhardt, F. Yeast (2006) [Pubmed]
The purine-cytosine permease gene of Saccharomyces cerevisiae: primary structure and deduced protein sequence of the FCY2 gene product. Weber, E., Rodriguez, C., Chevallier, M.R., Jund, R. Mol. Microbiol. (1990) [Pubmed]
The isolation and characterization of Saccharomyces cerevisiae mutants that constitutively express purine biosynthetic genes. Guetsova, M.L., Lecoq, K., Daignan-Fornier, B. Genetics (1997) [Pubmed]
New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants. Wagner, R., Straub, M.L., Souciet, J.L., Potier, S., de Montigny, J. J. Bacteriol. (2001) [Pubmed]
In vivo and in vitro studies of the purine-cytosine permease of Saccharomyces cerevisiae. Functional analysis of a mutant with an altered apparent transport constant of uptake. Brèthes, D., Chirio, M.C., Napias, C., Chevallier, M.R., Lavie, J.L., Chevallier, J. Eur. J. Biochem. (1992) [Pubmed]
Detection of purine cytosine permease of S. cerevisiae: use of antibodies against a synthetic peptide corresponding to a predicted sequence in the N-terminal domain of the protein. Grandier-Vazeille, X., Neaud, V., Geoffre, S. Biochem. Biophys. Res. Commun. (1993) [Pubmed]
In vivo phosphorylation of the purine/cytosine permease from the plasma membrane of the yeast Saccharomyces cerevisiae. Pinson, B., Pillois, X., Brèthes, D., Chevallier, J., Napias, C. Eur. J. Biochem. (1996) [Pubmed]

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AGOS_ADL074W	Ashbya gossypii ATCC 10895
KLLA0C08283g	Kluyveromyces lactis NRRL Y-1140
MGG_00158	Magnaporthe oryzae 70-15
FCY22	Saccharomyces cerevisiae S288c
FCY21	Saccharomyces cerevisiae S288c

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