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Svinarich, D.M. et al.

Svinarich, Zaher, Holmdahl, Chegini, Gonik, Diamond,

Adhesion development and the expression of endothelial nitric oxide synthase.

OBJECTIVE: This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions. METHODS: Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and beta-actin mRNA levels. RESULTS: eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were < or = 3.12 x 10(-4), < or = 3.12 x 10(-4), 6.24 x 10(-4) and 2.5 x 10(-3) attomoles/microl, respectively. Beta-actin mRNA levels for all tissues were between 1.25 x 10(-1) and 6.25 x 10(-2) attomoles/microl. CONCLUSION: eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.[1]

References

Adhesion development and the expression of endothelial nitric oxide synthase. Svinarich, D.M., Zaher, F.M., Holmdahl, L., Chegini, N., Gonik, B., Diamond, M.P. Infectious diseases in obstetrics and gynecology. (2001) [Pubmed]

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